1 / 28

Pseudomonas Identification

Pseudomonas Identification. Done By : Omniya Tayem Dr: Adbelraouf Elmanama Mohamad Laqan. Structure & Physiology. Pseudomonas. * Gram _ Negative rods. Gram negative rod. Motile with polar flagella *.

raleigh
Télécharger la présentation

Pseudomonas Identification

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Pseudomonas Identification Done By : Omniya Tayem Dr: Adbelraouf Elmanama Mohamad Laqan

  2. Structure & Physiology Pseudomonas • * Gram _ Negative rods Gram negative rod

  3. Motile with polar flagella *

  4. Motility positive

  5. Obligate aerobe

  6. Oxidase _ positive *

  7. Do not ferment Carbohydrates

  8. Resistant to multiple drugs

  9. Majority of human infections _ P. aeruginosa

  10. Common in the environment . Water . Air .Soil

  11. Very simple nutritional requirements Many organic compound used as C & N sources , but only afew carbohydrates by oxidative metabolism

  12. Some strains appear mucoid due to Polysaccharide Capsule

  13. Some produce diffusible pigments Pyocyanin _ nonfluorescent bluish pigment Pyoverdin_ Fluorescent greenish pigment Pyorubin & Pyomelanin

  14. Fluorescent Pigments White Light source Black Light source

  15. Pseudomonas . aeruginosa

  16. Pseudomonas aeruginosa colonies on Agar

  17. Pseudomonas . aeruginosa

  18. Identification of P. Aeruginosa Usually based on colonial morphology , B- hemolysis , Oxidase positivity , The presence of characteristic pigments & Sweet odor & Growth at 42c

  19. Identification Of Pseudomonas Pigments* Pyocyanin (blue _ green ) Fluorescein (green _yellow_fluorescent ) Pyorubin (red _ brown )

  20. Culures have fruity smell *

  21. Biochemical reactions * .Gelatine hydrolysis .Starch hydrolysis .Carbohydrates use Trehalose (O_F medium) .Flourescent Pigment

  22. Results

  23. P. aeruginosa Gelatin hydrolysis +/ - ( variable result ) Starch hydrolysis + / - ( variable result ) Acid Aerobically from D_ Trehalose ( negative ) Fluorescent pigment ( positive ) Non Fluorescent pigment ( positive )

  24. P .fluorescens Gelatin hydrolysis ( positive ) Starch hydrolysis ( negative ) Acid aerobically from D _ Trehalose (positive) Fluorescent pigment ( negative ) Non fluorescent pigment (positive )

  25. P. Saccharophila Gelatin hydrolysis (P) Starch hydrolysis (P) Acid aerobically from D_ Trehalose (P ) Fluorescent pigment ( N ) Non fluorescent pigment ( N )

  26. P. Putida Gelatine hydrolysis ( negative ) Starch hydrolysis (negative ) Acid aerobically from D_ Trehalose (N ) Fluorescent pigment ( P ) Non fluorescent pigment ( P )

  27. The End

More Related