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Ribosomal RNA ( rRNA ) Genes

Ribosomal RNA ( rRNA ) Genes. Laura McCoy. Background Information. rRNA genes are a multi-gene family Located in the nucleolus of the cell Genes are found in tandem arrays rRNA plus ribosomal proteins form ribosomes Mitochondria and chloroplasts have their own rRNA genes. Types.

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Ribosomal RNA ( rRNA ) Genes

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  1. Ribosomal RNA (rRNA) Genes Laura McCoy

  2. Background Information rRNA genes are a multi-gene family Located in the nucleolus of the cell Genes are found in tandem arrays rRNA plus ribosomal proteins form ribosomes Mitochondria and chloroplasts have their own rRNA genes

  3. Types http://www.mun.ca/biology/scarr/MGA2_03-23.html In prokaryotes: 23S, 5S,16S In eukaryotes: 28S, 5.8S, 5S, 18S

  4. Search for Blueberry rRNA genes Find orthologs in closely related species (Vitis Vinifera and Fragaria Vesca) Blast ortholog sequences against blueberry genome Analyze hits

  5. VitisVinifera • 18S (partial sequence) • GQ849399: 6 hits (454 sequence) • 16S- most likely chloroplast rRNA • AJ639615: 20 hits (454 sequence) • AF143264: 6 hits (454 sequence) • Mitochondria (complete genome) • FM1798380: 500 hits (454 sequence) **note: sequences found on rRNA database: http://www.arb-silva.de/search/

  6. FragariaVesca • EU024825: working draft sequence • 8 unordered unordered pieces • 43 hits (454 sequence) **note: sequence found on rRNA database: http://www.arb-silva.de/search/ • 5S • No hits found (454 or Illumina sequences) • 23S • No hits found (454 or Illumina sequences) **note: sequences found on http://www.strawberrygenome.org/

  7. Conclusion • Conservation of rRNA gene sequences between closely related species • 18S, chloroplast, mitochondria • Sequences of all rRNA genes in strawberry and grape are not available • 28S, 5S, 5.8S

  8. References Stutz, E., and Noll, H. 1967. Characterization of cytoplasmic and chloroplast polysomes in plants: evidence for three classes of ribosomal RNA in nature. Biochemistry. 57: 774-781. Velasco et al. 2007. A High Quality Draft Consensus Sequence of the Genome of a Heterozygous Grapevine Variety.

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