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Epitope Tagging of Ribosomal Proteins: Techniques and Applications

This document explores the methodologies and applications of epitope tagging in ribosomal proteins. Focusing on the use of restriction enzymes such as SstI, XbaI, NcoI, BamHI, EcoRI, and HindIII, it details techniques for tagging at both the N- and C-termini. The importance of the CaMV 35S promoter, nptII for selection, and r-protein construction are highlighted. This research provides insights into generating tagged ribosomal proteins for effective characterization and study of protein function in various biological systems.

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Epitope Tagging of Ribosomal Proteins: Techniques and Applications

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  1. EPITOPE TAGGING OF RIBOSOMAL PROTEINS

  2. SstI XbaI NcoI BamHI EcoRI HindIII ATG STOP RB nptII CaMV 35S CaMV 35S Ω HF r-protein OCS LB p35S:HF-RP BamHI XbaI SstI NcoI EcoRI HindIII ATG STOP RB nptII CaMV 35S CaMV 35S Ω r-protein FH OCS LB p35S:RP-FH A)N-terminus tagging B)C-terminus tagging

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