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Thiol quantification

Thiol quantification. Professor ： Chau , Lai-Kwan Student ： Liu, Wan-Chun Date ： 2013/11/08. Method. 2% MPDMS, or 2% MPTMS. plasma. 0 .004mM DTNB. Glass slide. UV. 12hr. 15min. 16hr. Optimization - concentration of DTNB. Mechanism of E llman’s reaction.

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Thiol quantification

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  1. Thiol quantification Professor：Chau, Lai-Kwan Student：Liu, Wan-Chun Date：2013/11/08

  2. Method 2%MPDMS, or 2% MPTMS plasma 0.004mMDTNB Glassslide UV 12hr 15min 16hr

  3. Optimization - concentration of DTNB

  4. Mechanism of Ellman’s reaction

  5. Verify by strong/weak reducing agents Strong reductant : DTT Weak reductant: 2-mercaptoethanol

  6. DTNB reacts with MPTMS/MPDMS

  7. Standard curve (r2=0.998)

  8. Concentration of thiols on glass surface

  9. Thiolcoverage on glass surface 5 cm (SH/nm2) 0.2 cm 0.8 cm

  10. Thiol coverage on glass surface 0.7 nm 0.4nm

  11. Future work • Gold coverage on MPDMS/MPTMS modified glass surface.

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