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Biology 201

Biology 201. Introduction to the Microscope. Laboratory Safety and Etiquette. Cabrillo College Department of Biology. Laboratory Rules. No eating or drinking in labs Do not enter halls; remain only in classroom Clean up after yourself. Laboratory Etiquette.

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Biology 201

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  1. Biology 201 Introduction to the Microscope

  2. Laboratory Safety and Etiquette Cabrillo College Department of Biology

  3. Laboratory Rules • No eating or drinking in labs • Do not enter halls; remain only in classroom • Clean up after yourself

  4. Laboratory Etiquette • Clean your personal workspace after use • Wipe down benches with disinfectant before and after use • Ensure that no trash is left behind • No trash in the sinks • Push in chairs • Place lab items back on carts and trays

  5. Laboratory Safety Outline 1. Laboratory Hygiene 2. Sharps Safety 3. Safety Equipment 4. Fire Safety 5. Chemical Safety 6. Biohazard Safety

  6. 1. Laboratory Hygiene • Avoid cross contamination • Do not touch self, faucets, doorknobs, notebooks, pens etc. with gloves on. • Clean and disinfect your workspace • Scrub hands thoroughly before leaving lab

  7. 2. Sharps Safety • Sharps include • Razor and scalpel blades • Needles and pins • Microscope cover slips • Broken glass • Place sharps in designated, labeled sharps boxes • Larger broken glass pieces go into designated glass box (ask for help) • NO SHARPS OR GLASS IN THE TRASH

  8. 3. Safety Equipment • Shower and eyewash (15 min. soak) • Do not hesitate to use this equipment if necessary • First aid kit

  9. 4. Fire Safety • Never leave flames unattended • Do not use flammables near ignition sources • Fire Extinguishers • Pull ring, aim at fire’s base, squeeze handle, sweep back and forth (evacuate if >1m3) • Fire Blanket • Fire alarm pulls

  10. 5. Chemical Safety • HAZMAT is short for “Hazardous Material” ~Any material that would be a danger to life or the environment if released without precautions • Dispose of chemical waste in provided containers • DO NOT pour chemical wastes down the drain • Wear gloves and safety glasses when appropriate • Follow the instructor’s directions when dealing with any chemical

  11. 6. BIOHAZARD Safety • BIOHAZARD is short for Biological Hazard ~Any biological substance or substances that may pose a threat to the health of a living organism, particularly humans • Biohazards include, but are not limited to: Human cells, bodily fluids, and bacterial cultures • Biohazard sharps must be placed into the biohazard Sharps container • Solid Biohazard materials must be sealed in a biohazard bag, and then placed into the biohazard bin for professional disposal

  12. History of the Microscope • Anton van Leeuwenhoek (1676) was first to see microorganisms

  13. History of the Microscope • Robert Hooke proposed “cell theory” and invented primitive compound microscopes

  14. A Compound Microscope

  15. Rules for microscope protection • Cary microscope upright with two hands • Clean lenses with lens paper ONLY • Always begin with lowest power objective • Focus downwards • Never use course focus with the 10x, 40x or 100x objective • When returning to cabinet: remove last slide, lower stage, put 4x objective in place, turn dimmer down to 1, wrap cord, place in cabinet with eyepieces facing inward

  16. Slides we will look at today • Letter “e” • Colored threads • Elodea plant • Cheek cells • Blood smear

  17. This slide is brought to you by the letter “e” (40x objective)

  18. Slide of thread with 40x objective

  19. Slide of elodea with 10x and 40x objectives

  20. Cytoplasmic streaming of chloroplasts inside elodea http://www.youtube.com/watch?v=PFtzs_cUddI

  21. Slide of elodea with 40x objective in Sodium chloride

  22. Properties of Light

  23. Properties of Light • Oil matches the refraction index of glass

  24. Slide of human cheek cells with 40x objective

  25. Slide of human blood cells under oil immersion (100x objective)

  26. Clean up reminder

  27. The end

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