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AtSUM1 MSA--------NQEEDKKPG-DGGAHINLKVKGQDGNEVFFRIKRSTQLKKLMNAYCDRQ

Supplemental Fig. 1. AtSUM1 MSA--------NQEEDKKPG-DGGAHINLKVKGQDGNEVFFRIKRSTQLKKLMNAYCDRQ PtSUM1 MSEAT----GQPQEEDKKPN-DQSAHINLKVKGQDGNEVFFRIKRSTQLKKLMNAYCDRQ PtSUM2 MSGAT----GQPQEEDKKPN-DQSAHINLKVKGQDGNEVFFRIKRSTQLKKLMNAYCDRQ

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AtSUM1 MSA--------NQEEDKKPG-DGGAHINLKVKGQDGNEVFFRIKRSTQLKKLMNAYCDRQ

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  1. Supplemental Fig. 1 AtSUM1 MSA--------NQEEDKKPG-DGGAHINLKVKGQDGNEVFFRIKRSTQLKKLMNAYCDRQ PtSUM1 MSEAT----GQPQEEDKKPN-DQSAHINLKVKGQDGNEVFFRIKRSTQLKKLMNAYCDRQ PtSUM2 MSGAT----GQPQEEDKKPN-DQSAHINLKVKGQDGNEVFFRIKRSTQLKKLMNAYCDRQ PtSUM3 MSGVT----GQPQEEDKKPN-DQSAHINLKVKGQDGNEVFFRIKRSTQLKKLMNAYCDRQ PtSUM4 MSASAGGGGGGGQEEDKKPGGDQSAHINLKVKGQDGNEVFFRIKRSTQLRKLMTAYCDRQ ** *******. * .*************************:***.****** AtSUM1 SVDMNSIAFLFDGRRLRAEQTPDELDMEDGDEIDAMLHQTGGSGGGAT--A PtSUM1 SVEINSIAFLFDGRRLRGEQTPDELDMEDGDEIDAMLHQTGGAV-KASDYA PtSUM2 SVEFNSIAFLFDGRRLRGEQTPDELDMEDGDEIDAMLHQTGGAV-KTS--N PtSUM3 SVEFNSIAFLFDGRRLRGEQTPDELDMEDGDEIDAMLHQTGGAM-KTS--N PtSUM4 SVEFNSIAFLFDGRRLRGEQTPDELDMEDGDEIDAMLHQTGGGH-ASL--D **::*************.************************. : Suppl. Fig. 1. SUMO sequence alignments of translated Arabidopsis AtSUM1 (At4g26840), PtSUM1 (POPTR_0002s21690), PtSUM2 (POPTR_0002s21680), PtSUM3 (POPTR_0014s15650) and PtSUM4 (POPTR_0014s18990).

  2. Supplemental Fig. 2 Gene ModelOriginal ModelTotal # EST hits (# with 99-100% identity) POPTR_0082s00230 eugene3.02100027 17(1) POPTR_0010s01730 13(3 from elicited cells, 2 different cDNAs) POPTR_0008s22250 7 (1) POPTR_0004s06880 0 (2) POPTR_0004s07000 40(30 from forest tent caterpillar, 15 different cDNAs POPTR_0002s10590 eugene3.00020973 17(1) POPTR_0007s03240 eugene3.00070310 5 (0) POPTR_0005s17970 eugene3.00050619 13(2) POPTR_0010s04980 1 (1) POPTR_0017s05090 3 (0) POPTR_0017s05000 1 (1) POPTR_0015s10250 1 (0) POPTR_0010s04970 1 (1) POPTR_0010s04980 0 (0) Suppl. Fig. 2. SUMO protease gene models and Populus ESTs at NCBI. The gene models designated with “original models” from the original annotation of the poplar genome (Tuskan et al. 2006) were included in the poplar gene atlas (Quesada et al. 2008) and the transcript abundance from each of these genes is shown in Fig. 2 b of this paper.

  3. Supplemental Fig. 3 AtESD4 IFVPIHRGVHWTLAVINNRESKLLYLDSLNG-VDP---MILNALAKYMGDEANEKSGKKI AtULP1a IFIPIHMNIHWTLAVINIKDQKFQYLDSFKG-REP---KILDALARYFVDEVRDKSEVDL AtULP1b IFVPIHIDIHWTLGVINNRERKFVYLDSLFTGVGH---TILNAMAKYLVDEVKQKSQKNI POPTR_0008s22250 IFVPVHQEIHWCLAVINKKDQKFQYLDSLKG-RDS---RVLESLAKYYAEEVKDKSKKDI POPTR_0004s06880 LFIPLCLENHWILICVDMEKRGLLWLDSLNS-PPDAHHTEKATISEWLVKHLLPVLGYR- Danio LLIPIHLEVHWSLVSVDIKRRSITYFDSQRT-LNR---RCPKHIFKYLQAEAMIKEKRDF Bos LLIPIHLEVHWSLISVDVRRRTITYFDSQRT-LNR---RCPKHIAKYLQAEAVKKDRLDF Equus LLIPIHLEVHWSLISVDVRRRTITYFDSQRT-LNR---RCPKHIAKYLQAEAVKKDRLDF Homo LLIPIHLEVHWSLISVDVRRRTITYFDSQRT-LNR---RCPKHIAKYLQAEAVKKDRLDF :::*: ** * :: . : ::** : .: . AtESD4 DANSWDMEFVEDLPQQKNGYDCGMFMLKYIDFFSRGLGLCFSQ AtULP1a DVSRWRQEFVQDLPMQRNGFDCGMFMVKYIDFYSRGLDLCFTQ AtULP1b DVSSWGMEYVEERPQQQNGYDCGMFMLKYIDFYSRGLSLQFSQ POPTR_0008s22250 DVSNWEREFVEDLPEQQNGYDCGVFMIKYADFYSRGIGLCFGQ POPTR_0004s06880 NSQQWKFLQLKDIPYQTNRIDCGIFVMKYADCLAHCDHFPFTQ Danio -LTGWKGFFKMNVGRQNNDSDCGAFVLQYCKCLALGQPFSFSQ Bos -HQGWKGYFKMNVARQNNDSDCGAFVLQYCKHLALSQPFSFTQ Equus -HHGWKGYFKMNVARQNNDSDCGAFVLQYCKHLALSQPFSFTQ Homo -HQGWKGYFKMNVARQNNDSDCGAFVLQYCKHLALSQPFSFTQ * : * * *** *:::* . : : * * Suppl. Fig. 3. SUMO protease alignments showing the conserved H/D/C catalytic triad (boxed residues) after TCOFFEE alignment of Arabidopsis ESD4 (At4g15880), Arabidopsis ULP1a (At3g06910), Arabidopsis ULP1b (At4g00690), Populus (POPTR_0008s22250), Populus (POPTR_0004s06880), Danio (NP_001077311), Bos (XP_001790522), Equus (XP_001504827) and Homo (EAW90170).

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