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Photoinduced Cytotoxicity of K562 Cells Using Acridine Orange and Propidium Iodide Staining

This study examines the photoinduced cytotoxicity effects on K562 cells using fluorescence microscopy. Cells were stained with Acridine Orange (AO) and Propidium Iodide (PI) to differentiate between live (green) and dead (red) cells under various conditions. K562 cells were incubated in full medium with a 500 nM sensitizer for 24 hours in darkness. The middle panel shows cells incubated in the dark, while the right panel displays cells irradiated with red LED light at 625 nm for 4 hours, followed by a 20-hour dark incubation at 37°C.

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Photoinduced Cytotoxicity of K562 Cells Using Acridine Orange and Propidium Iodide Staining

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  1. Bilkent Üniversitesi Photoinduced cytotoxicity Fluorescence microscope images of acridine orange (AO) and propidium iodide (PI) stained K562 cells, incubated in full medium with 500 nM sensitizer 3 in dark (the middle panel); irradiated with red LED at 625 for 4 hours followed by 20 hr incubation in dark at 37 oC in the presence of 500 nM sensitizer 3 (right panel); and incubated in dark for 24 hr (left panel). Live cells were preferentially stained with AO (green) and dead cells with PI (red) due to increased cellular permeability.

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