1. Ogami_Fig. S1 pHA-CMV5- CPEB 0 Time (h) 0 1.5 3 oligo(dT)/ RNase H GST-CAT mRNA BGG c-myc 3’UTR mRNA 1 2 3 4 5 6 7 8 9 10

2. Ogami_Fig. S2 A MS2-HA -CPEB ( ) ( ) MS2-HA HA-CPEB ( ) (kb) 6 2 4 6 8 0 2 4 6 8 0 8 0 2 4 Time (h) 1.5 EGFP mRNA 1.0 BGG(1-39) MS2bs mRNA 0.8 0.6 14 15 16 1 2 3 4 5 6 7 8 9 10 11 12 13 17 B C MS2-HA-CPEB 100 MS2-HA HA-CPEB (kDa) 89 RNA level (%) MS2-HA-CPEB 10 HA-CPEB 63 (WB: anti-HA) MS2-HA MS2-HA-CPEB GAPDH 35 (WB: anti-GAPDH) HA-CPEB 1 0 2 4 6 8 Time (h)

3. Ogami_Fig. S3 pCMV-5XMyc-PARN CPEB CPEB3 CPEB CPEB3 pCMV-5XFlag- anti-Flag anti-Flag anti-Flag IP (kDa) 5XMyc-PARN 93 (WB: anti-Myc) 5XFlag-CPEB3 93 5XFlag-CPEB 62 (WB: anti-Flag) 1 2 3 4 5 6

4. Ogami_Fig. S4 B A mature (a-c) pre (b-c) Maker RT