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Super-Resolution Optical Microscopy

Super-Resolution Optical Microscopy

Super-Resolution Optical Microscopy. Bo Huang Light Microscopy May 10, 2010. l. d ?. 2 NA. Naked eye: ~ 50-100 ? m. 1595, Zaccharias and Hans Janssen First microscope, 9x magnification. Antony Van Leeuwenhoek (1632-1723), 200x. Ernst Abbe (1840-1905)

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1.58k views • 50 slides
Stochastic Optical Reconstruction Microscopy (STORM)

Stochastic Optical Reconstruction Microscopy (STORM)

Stochastic Optical Reconstruction Microscopy (STORM). Finding Out the Position of a Molecule. FIONA. ? ? ? PSF / N 1/2. Thompson at al., Biophys. J. , 2002. Yildiz et al., Science , 2003. Localization by sequential photobleaching. or QD blinking. A few molecules.

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917 views • 24 slides
Stochastic Optical Reconstruction Microscopy (STORM)

Stochastic Optical Reconstruction Microscopy (STORM)

Stochastic Optical Reconstruction Microscopy (STORM). Finding Out the Position of a Molecule. FIONA. ? ? ? PSF / N 1/2. Thompson at al., Biophys. J. , 2002. Yildiz et al., Science , 2003. Localization by sequential photobleaching. or QD blinking. A few molecules.

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631 views • 24 slides
780/60

780/60

X. 525/50. 505. Violet (405 nm). 450/50. FACSCanto II Configuration. Alexa 430 V500 CFP Qdot 525 Qdot 545 Cascade Yellow Lucifer Yellow Pacific Orange. PerCP PerCP/Cy5.5 PE/Cy5 7AAD PE/Cy5.5. APC Alexa 632 Alexa 647 Cy5. APC/Cy7 APC/H7 APC/Alexa 750 Cy7. PE PI.

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184 views • 1 slides
Blue (488 nm)

Blue (488 nm)

X. 525/50. 505. Violet (405 nm). 450/50. LSR II Configuration. *PE-Alexa 610 (630/30). *YFP if doing w/ GFP (540/20 525LP). *Hoechst Red (635LP 670LP). APC Alexa 633 Alexa 647 Live/Dead Far Red. AmCyan CFP BD Horizon V500 Live/Dead Yellow. PI 1 PE-Texas Red mCherry

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151 views • 1 slides

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