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EMBO Practical course on Quantitative FRET, FRAP and FCS Live-cell FRET

EMBO Practical course on Quantitative FRET, FRAP and FCS Live-cell FRET

EMBO Practical course on Quantitative FRET, FRAP and FCS Live-cell FRET. ZMBH. Victor Sourjik ZMBH, University of Heidelberg. Measuring FRET in vivo. Define the goal. Choose fluorescent labels. Choose your method. Get data !. I. Goals of in vivo FRET measurements.

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827 views • 38 slides
Single molecules & Photo-physics

Single molecules & Photo-physics

Single molecules & Photo-physics. Dirk-Peter Herten Heidelberg University. EMBO Course: F-Techniques Heidelberg, 23. -27.9.2009. Molecules. Models. Human models. Modeling. Individual. Individual. Model. Average. Individual. Ensemble = Average. Model. deduce. Why single molecules?.

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617 views • 36 slides
V12: Folding of Single Proteins

V12: Folding of Single Proteins

V12: Folding of Single Proteins. Why study single proteins? How do proteins spontaneously find their native structure? Theoretical studies ? overall downhill funnel-shaped energy surface. Surface is complex and rugged ? folding behavior of individual molecules may differ widely!.

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432 views • 29 slides
Protein structure

Protein structure

Protein structure. Primary structure Secondary structure Tertiary structure. Primary structure of polypeptides. The primary structure of a protein is specified by the nucleotide sequence of the corresponding gene.

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416 views • 21 slides
BDNA/ZDNA FRET efficiency

BDNA/ZDNA FRET efficiency

BDNA/ZDNA FRET efficiency. SNU Compbio. ? ? ?. Introduction : Z-DNA. Is this a proper model for transcription process?. Introduction : Z-DNA. Supercoiling of the DNA template during transcription, LEROY F. LIU & JAMES C. WANG, June 17, 7024 (1987) , PNAS

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341 views • 20 slides
FRET(Fluorescent Resonance Energy Transfer)

FRET(Fluorescent Resonance Energy Transfer)

FRET(Fluorescent Resonance Energy Transfer). The problem. The use of fluorescent probes (ex. GFP) permits direct observation of the dynamic properties of specific proteins in live cells .

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763 views • 18 slides
Flow Cytometric Analysis of FRET to Study the Interaction Between CFP- and YFP-Tagged Proteins

Flow Cytometric Analysis of FRET to Study the Interaction Between CFP- and YFP-Tagged Proteins

Flow Cytometric Analysis of FRET to Study the Interaction Between CFP- and YFP-Tagged Proteins. David Stepensky. Classical pathway of major histocompatibility complex (MHC) class I antigen processing, loading, and presentation. Groothuis et al, Immunol Rev 2005. Objectives.

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394 views • 12 slides
Fluorescence

Fluorescence

Fluorescence. Energy diagram. Energy diagram. Quantum Yield - Q. Rate of depopulation. Red shift. Fluorimeter. Time resolved experiment . Spectrofluorimeter. Frequency domain (phase/modulation) . Excitation/ emmission spectrum :. Fluorophores. Intrinsic fluorophores. Extrinsic probes.

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414 views • 9 slides
N Akyuz et al. Nature 000 , 1-5 (2013) doi:10.1038/nature12265

N Akyuz et al. Nature 000 , 1-5 (2013) doi:10.1038/nature12265

FRET efficiency changes reflect relative orientations of the transport domains. N Akyuz et al. Nature 000 , 1-5 (2013) doi:10.1038/nature12265.

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134 views • 1 slides

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