'Inclusion bodies' diaporamas de présentation

Herpesviridae Family

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VIROLOGY

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Bacteria I

Bacteria I. General Nature. 1) Prokaryotic 2) Single celled (some say multicellular) 3) Microscopic in size 4) Cell Wall usually present (varies, but contains contains carbohydrate called _____________. Typical Bacterial Cell (Prokaryotic). Bacterial Colonies growing on Agar plates.

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Structure of Prokaryotic & Eukaryotic Cells

Structure of Prokaryotic & Eukaryotic Cells. Review of Prokaryotic & Eukaryotic Cells. Nucleus vs nucleoid DNA : circular vs linear, presence of histones Membranous organelles Cell wall-peptidoglycan Cell division: binary vs mitosis Ribosomes: 70S vs 80S Cytoskeleton. Shape. Cocci

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Virology practice

Virology practice. Cultivation of viruses. Since viruses are obligate intracellular parasites they have to be grown in living cells. There are three systems for their cultivation: 1- tissue culture. 2- embryonated eggs. 3- intact animals. 1- tissue cultures:

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Retroviridae

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Prokaryotic Cell Structure

Prokaryotic Cell Structure. A. Generalized Structure 1. Cell Appendages A) Flagella 1) Functions in movement of the cell 2) 3 components. Prokaryotic Cell Structure. a) Filament i) Whip-like, helical structure b) Hook i) Holds the filament

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Herpes viruses

Herpes viruses. Introduction. Leading cause of human viral disease, second only to influenza & cold viruses. Capable of causing overt disease or remaining silent for many years only to be reactivated, e.g. shingles

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More is Not Always More: Unstable Repeat Expansion Disorders

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Chlamydiae

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Objectives for NS 3:

Objectives for NS 3:. You should be able to define, describe pathogenesis, list lesions and know how to diagnose the following conditions:. Infectious diseases of CNS. Portal of entry. Blood Nerves Paracranial and paravertebral infections (sinuses, ear, bones et cet). Meningitis.

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Basics of Cell Culture

Basics of Cell Culture. Part 2: Choosing a Host Cell. Overview. Kinds of cells used in industry Advantages Disadvantages Large scale cell growth issues Sterility Cleanliness Consistency. Cells Used in Bioprocessing. Different options Choice based on many variables

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MICR 420

MICR 420. Emerging and Re-Emerging Infectious Diseases Lecture 7: C. trachomatis Dr. Nancy McQueen & Dr. Edith Porter. C. trachomatis. The genus Chlamydia Morphology Growth and metabolic characteristics Virulence factors Diseases Diagnosis Direct antigen test Culture PCR

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Canine distemper

Canine distemper. By: Melissa McGarity & Aly Martinez. Etiology. Distemper was once thought to be caused by neglect, contaminated food, and anti-hygienic conditions, however we now know that canine distemper is a virus that is shed in body secretions. Pansystemic disease. History.

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Towards an Asthma Vaccine: Scalable Process for the Purification of a Latex Allergen Protein

Towards an Asthma Vaccine: Scalable Process for the Purification of a Latex Allergen Protein. Presented by : Anushi E. Kulasiri. The Problem. 80% of occupational asthma in the healthcare industry is caused by latex allergies.

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Renaturation of Recombinant Proteins

Recombinant proteins expressed in bacteria often form inclusion bodies, especially when they are expressed at high levels. Creative BioMart retrieves expressed GST fusion protein in soluble form after lysis and extraction procedures.

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refolding of recombinant proteins

Recombinant protein expression in bacteria often results in the formation of both inactive and insoluble protein that accumulates as intracellularprotein aggregates called inclusion bodies [1]. It has beenshown that 70-80% of recombinant proteins expressed in E.coli are as inclusion bodies [2]. This is probably due to the independence of the protein type in bacterial systems. In cases of expression of eukaryotic proteins, which usually contain cysteines that are prone to form disulfide bonds in the nativestate, the bacterial system maynot support the appropriate pairing of disulfide bonds in the newly-produced protein thus leads to the presence of insoluble protein pellets [3].

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an introductionforbacterial fermentation process

bacterial fermentation process bacterial fermentation process bacterial fermentation process

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a -syn

Supplementary Figure 1. A. B. merge. merge. a -syn. a -syn. ThioS. ThioS. a -syn. ubiq. merge. ubiq. merge. a -syn. merge. a -syn. a -syn. merge. hsp70. hsp70. a -syn. a -syn. merge. merge. 20s. 20s.

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