'Lac operon' diaporamas de présentation

Chapter 18 Gene Expression. Gene Expression and Metabolic Pathways. A gene is being “expressed” or “activated” when a protein is being made Some are expressed for a time and then turned off to conserve cell resources Controls of Metabolic Pathways

AP Biology Final: Key terms to Review. Kreb Cycle Hardy-Weinberg Mitosis vs. Meiosis (phases) Fungi-characteristics Properties of Proteins Lac operon system (E. coli) Peptide bonds Enzyme activities

Footprinting DNA-Protein Interactions. Powerful and fairly rapid methods for mapping where and how proteins bind tightly to DNA 2 ways: DNAse I footprinting DMS footprinting. DNAse I Footprinting. Prepare end-labeled DNA. Bind protein.

Regulating Gene Expression. Homework #2 is posted and due 10/18 Exam key is posted. Combinations of 3 nucleotides code for each 1 amino acid in a protein. Exons are a very small part of DNA.

OUTLINE 3 Control of Gene Expression in Prokaryotes A. Regulatory proteins B. The operon model C. Examples 1. the lac operon (substrate induction) 2. the tryp operon (end product repression) 3. the lac operon (positive control). Fig. 18.21. Negative regulation:

Genetics - Ch 10. Molecular Mechanisms of Gene Regulation. Control of Gene Expression. DNA -------------> RNA ------------> protein . transcriptional control. translational control.

Lecture 6. 第 3 章 3.1 为什么使用 RNA 3.2 转录机理 3.3 原核生物基因 表达调控 3.3.1 协同调控 3.3.2 乳糖操纵子 3.3.3 色氨酸操纵子 3.3.4 Ara 与 Gal 3.4 实验研究. Chapter 3 3.1 Why Use an RNA Intermediate? 3.2 Mechanism of Transcription 3.3 Regulation of Gene Expression in Prokaryotes

Gene Regulation. How does your body know when to make proteins? Unit 4 – Chapter 12-5. No Lactose Present:. Is lactase made? WHY? NO, because the repressor protein is blocking RNA polymerase from copying the gene for lactase. With Lactose Present:.

Regulation of eukaryotic gene sequence expression. Lecture 6. Introduction. Difference between eukaryotic and prokaryotic DNA Brief Overview of Eukaryotic “gene” expression regulation. Regulation at the transcription level Post transcriptional regulation Alternative Splicing

Announcements. Homework for Chapter 7 Part 3 due Friday If you have VUU sponsored event-turn it in by email (scan it...via Genius Scan App) If you aren’t in class to get the homework…download it from MyVUU or class website. You are expected to turn it in by the due date.

Unit #3 Schedule:. Previously: Sanger Sequencing Central Dogma Overview Mutation Transcription , RNA Processing, Translation Last Class: Central Dogma Sculpting Today: Regulation of Gene Expression + Trivia StudyNotes 9 Due Tutorial (Apr 5) Review (Apr 9) EXAM 3 (Apr 11 )

Unit 13: Prokaryotic Gene Expression Lesson 2: lac vs trp operons. Please Make Sure your final protein structure questions are turned in. Start do-now silently. Not all proteins need to be made all the time. Some proteins are made infrequently (specific antibodies towards diseases)

Operons: Bacterial Gene Control. Operons. Bacterial cells A group of genes that work together Illustrate how genes expression (“on”) and repression (“off”) can be controlled. Operon components. Regulatory gene : C odes for production of the repressor Operator :

Gene Regulation. How does your body know when to make certain proteins? Unit 4 – Chapter 12-5. Gene Regulation Vocab. RNA Polymerase: Enzyme that makes an RNA copy of DNA lac Operon : a group of genes that operate together to regulate the production of lactase

Gene Regulation. Remember! All of your cells contain the exact same DNA. Your nerve cells do not look like or act like skin cells, so do they use the same genes? NO!. Gene Regulation. Genes are to be turned on/off based on whether they will be used or not!

Gene Regulation. Complied by Siti Sarah Jumali Room 14, level 3 ext2123. Overall process of transcription and translation. Gene Expression. An individual ’ s trillions of body cells all come from one cell (zygote).

Prokaryotic Gene Regulation:. Lecture 5. overview. Generic types of regulation control Regulation of the “sugar” lactose gene(s) for the bactria e. coli [ referred to as the lac operon] Regulation of the expression of the “amino acid” gene tryptophan in E. Coli. [try operon].

Positive Feedback and Bistability. BIOE 423: 2013. Stable state. Transient state. Stable state. Simulation of biochemical network. Stable steady state. Multiple stable states. Different starting points lead to different steady states. Positive Feedback. v1 = ? v2 = ? dS/dt = ?. v2.

A genome-wide perspective on translation of proteins. Dec 2012 Regulatory Genomics Lecturer: Prof. Yitzhak Pilpel. Teaching assistant: Idan Frumkin. idan.frumkin@weizmann.ac.il Submit Sunday at midnight. The Central Dogma of Molecular Biology Expressing the genome. RNA. Inactive DNA.

Prokaryotic Gene Regulation:. Lecture 5. Introduction. The two types of transcription regulation control in prokaryotic cells The lac operon an inducible regulatory feedback system The tryp operon a repressible regulatory feedback system. The role of bioinformatics analysis in such systems.