'Mrna mrna' diaporamas de présentation

Microarrays for Gene Expression Analysis

Microarrays for Gene Expression Analysis. Questions: What genes are expressed in this tissue under these conditions? What genes are expressed in my treated cells versus the control? What genes are expressed during the phases of the cell cycle?

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?????????. ?????????? ?????? ( Reverse dot hybridization) ?????????????????????? ( allele specific oligonucleotide, ASO) ??????? ASO ????? 20 bp ???????????????????????????????? ??????????????????? PCR ?????????????????????????? PCR ??????????? ? ???????????????

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Anatomy of the Gene

Anatomy of the Gene. MUPGRET Workshop June 17, 2004. 2003: 50 Year Anniversary of the Discovery of The DNA Double Helix. The famous DNA Double Helix paper was published in Nature in 1953!. What Is DNA?. DNA is a chemical contained in every cell of your body. Yes!. DNA is a Chemical!?.

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DNA Science

DNA Science. MU Plant Genome Research Experience for Teachers Workshop. Friedrich Miescher. First to extract DNA from nuclei (1869). First to ID DNA as a distinct molecule. Source of DNA was white blood cells (WBC). WBCs are commonly found in infected wounds. Friedrich Miescher (cont.).

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Lac Operon

Lac Operon. Lactose and Glucose. Much of the control of gene expression occurs at the transcriptional level Our understanding of transcriptional regulation comes from studies of enzyme induction in E.coli E. Coli exhibit an extremely sophisticated regulation of enzyme

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Methods for determining gene function

Methods for determining gene function. Study the effects of disrupting gene function ( ap3 mutants) Examine the expression of the gene (GUS, In situ). Methods for examining the expression of a gene. Reporter construct ( AP1::GUS ) Pro- easy, can be used to define promoter region

214 views • 8 slides