'Restriction endonuclease' diaporamas de présentation

Recombinant DNA Technology

Recombinant DNA Technology. replication. replication. transcription. transcription. translation. translation. Gene expression in vivo. DNA from different source. DNA Recomnbinant. DNA Recombination. Development of clone technique. 1. Restriction Enzymes and DNA Ligase Are Key

1.16k views • 93 slides

Recombinant DNA

Recombinant DNA. OVERVIEW OF RECOMBINANT DNA TECHNOLOGY. Recombinant DNA technology allows a DNA fragment from any source to be joined in vitro with a nucleic acid vector that can replicate autonomously in microorganisms.

594 views • 59 slides

I Recombinant DNA technology

I Recombinant DNA technology. 1 Restriction enzymes 2 Nucleic acid hybridization 3 DNA cloning 4 Viruses 5 DNA sequencing 6 Polymerase chain reaction. Restriction enzymes. Overview Restriction enzyme digestion Nomenclature Gel electrophoresis Restriction maps

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Digesting DNA with restriction endonuclease

Digesting DNA with restriction endonuclease Mix the DNA solution and reaction buffer (Total volume: Y ml) DNA solution X m l ( 5 m l) 10x reaction buffer 1/Y m l ( 1 m l) Restriction Enzyme 1 m l ( 1 m l) Add ddH 2 O to Y m l ( 3 m l)

594 views • 41 slides

Recombinant DNA

Recombinant DNA. OVERVIEW OF RECOMBINANT DNA TECHNOLOGY. Recombinant DNA technology allows a DNA fragment from any source to be joined in vitro with a nucleic acid vector that can replicate autonomously in microorganisms.

822 views • 40 slides

Recombinant DNA, Biotechnology, and Microbes

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Orientation Lab Safety and Restriction Enzymes

0. Orientation Lab Safety and Restriction Enzymes. Kabi Neupane, Ph.D. Leeward Community College. 0. Objectives. Familiarize with laboratory safety Learn about Restriction enzymes Perform a restriction digestion of Arabidopsis genomic DNA. 0. Laboratory Safety. Emergency procedures

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Routine Molecular Epidemiology for Enhanced Detection and Control of Foodborne Outbreaks

Routine Molecular Epidemiology for Enhanced Detection and Control of Foodborne Outbreaks. Lee H. Harrison, MD Associate Professor Departments of Epidemiology and Medicine University of Pittsburgh. What is molecular epidemiology in infectious diseases?.

298 views • 19 slides

NAJRAN UNIVERSITY College of Applied Medical Sciences

NAJRAN UNIVERSITY College of Applied Medical Sciences. General Microbiology Course Lecture No. 8. By. Dr. Ahmed Morad Asaad Associate Professor of Microbiology. Genotypic variations:

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Molecular diagnostics in congenital adrenal hyperplasia (21-hydroxylase deficiency )

Molecular diagnostics in congenital adrenal hyperplasia (21-hydroxylase deficiency ). Introduction.

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Spec statements: Fragments of DNA can be produced by

Spec statements: Fragments of DNA can be produced by Conversion of mRNA to cDNA , using reverse transcriptase. Cutting DNA at specific, palindromic recognition sequences using restriction endonucleases. The polymerase chain reaction (PCR)

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NAJRAN UNIVERSITY College of Applied Medical Sciences

NAJRAN UNIVERSITY College of Applied Medical Sciences. General Microbiology Course Lecture No. 9. By. Dr. Ahmed Morad Asaad Associate Professor of Microbiology. Genetic engineering

176 views • 6 slides