'Restriction endonucleases' diaporamas de présentation

Wednesday 4/9/14

Wednesday 4/9/14. AIM: How is DNA useful to forensic science? DO NOW: List at least 4 sources of DNA that could be extracted from a crime scene HOMEWORK: Text read pages 343-348. questions 9-11 pages364-365. DNA: deoxyribonucleic acid. It is a polymer

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Chapter 2

Chapter 2. Enzymes for Genetic Engineering. 2.1 Restriction endonucleases 2.2 DNA ligase 2.3 DNA polymerase 2.4 Other Enzymes 2.5 probe labeling techniques. ??? ???????? ???????? 1. ?????????????????????????????? 2 ???????????????????????????????????? ????? 1 ????????? 2 ????

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FROM GENE TO DNA TECHNOLOGY

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4.4 Biotechnological Tools and Techniques

4.4 Biotechnological Tools and Techniques. Recombinant DNA & Gel electrophoresis. Recombinant DNA. Cutting DNA fragments from different sources and recombining them together. Cutting DNA fragments from different sources and recombining them together

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Microbial Genetics

Microbial Genetics. A. Structure of DNA 1. discovered by James Watson and Francis Crick in 1953 2. stores genetic information for the production of proteins 3. composed of nucleotides A) a nucleotide is composed of a nitrogenous base, sugar, and phosphate. Microbial Genetics.

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Restriction Enzymes

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6.1 Biotechnological Tools and Techniques

6.1 Biotechnological Tools and Techniques. Recombinant DNA & Gel electrophoresis. Recombinant DNA. Cutting DNA fragments from different sources and recombining them together. Cutting DNA fragments from different sources and recombining them together. Purpose To investigate genetic disorders

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Introduction to Techniques

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G1 DNA Cloning: An Overview

Section G-I Gene Manipulation G1 DNA cloning: an overview G2 Preparation of plasmid DNA G3 Restriction enzymes. G1 DNA Cloning: An Overview. DNA cloning Sub-cloning DNA libraries Screening libraries. Vectors Plasmids Hosts Analysis of clone. E. S. E. E.

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Gene-specific Methylation Analyses

Gene-specific Methylation Analyses. Fade Gong & Nam Nguyen. Introduction. DNA methylation in mammals occurs at CpG sites, where it serves many functions: Self vs. non-self identification (such as in viral/bacterial infection) Target for further regulatory events; examples:

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Objective

de Lipathay et al. 2003. In situ exposure to low herbicide concentrations affects microbial population composition and catabolic gene frequency in an aerobic shallow aquifer. Appl. Environ. Microbiol . 69: 461-467. Objective.

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Exercise 4:

Exercise 4:. DNA. Announcements. Post Lab 4 and Pre Lab 5 are due by your next lab period. LNA: This weeks lab and next weeks go together. Be sure to write your procedures, and any changes made. It will not be due until the week of March 10.

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Gene structure DNA replication

Gene structure DNA replication. Figure 7.4A. Figure 7.5. The DNA double helix has grooves: a wide major groove and a narrow minor groove. - These provide DNA-binding proteins access to base sequences. DNA Structure. Figure 7.6. RNA differs from DNA: - Usually single-stranded

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Gel electrophoresis

Gel electrophoresis. L Mathias. Definition. Separation of DNA fragments according to size, based on movement through a gel medium when an electric field is applied. . DNA negatively charged. DNA cut up using restriction endonucleases. Make up the gel which the DNA will be put into.

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Gel electrophoresis

Gel electrophoresis. L Mathias. Definition. Separation of DNA fragments according to size, based on movement through a gel medium when an electric field is applied. . DNA negatively charged. DNA cut up using restriction endonucleases. Make up the gel which the DNA will be put into.

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Spec statements: Fragments of DNA can be produced by

Spec statements: Fragments of DNA can be produced by Conversion of mRNA to cDNA , using reverse transcriptase. Cutting DNA at specific, palindromic recognition sequences using restriction endonucleases. The polymerase chain reaction (PCR)

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Cloning DNA

Cloning DNA. May 4. Cloning DNA. the components vector (plasmids, bacteriophage) cutters (restriction endonucleases) joiners (ligase, topoisomerase) insert (DNA, mRNA->cDNA) host strains of E.coli. Restriction endonucleases. REs and methylases Type I, II, IIs, III enzymes

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RFLP Restriction Fragment Length Polymorphism

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Southern blot method

Southern blot method Restriction endonucleases are used to cut high-molecular-weight DNA strands into smaller fragments. The DNA fragments are then electrophoresed on an agarose gel to separate them by size.

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