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Bioinformatics and Molecular Modeling studies of Membrane Proteins

Bioinformatics and Molecular Modeling studies of Membrane Proteins. Shiva Amiri. Problem : Difficult to obtain high resolution crystallographic images of membrane proteins. Structure Determination. Unwin et.al , Nature , 26 June 2003. Getting there?.

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Preparation of High Quality Protein Crystals by

Standard Screen. Salt Screen. #. #. Hits. Hits. Precipitant. Precipitant. Salt. Salt. Buffer. Buffer. Like*. Like*. 75. 73. 11. 8. 30%(w/v) PEG 8000. 30%(w/v) PEG 4000. 0.2 M (NH 4 ) 2 SO 4. 0.2 M Li 2 SO 4. 0.1 M Cacodylate pH 6.5. 0.1 M Tris pH 8.5. H1 #15. H1 #17. 75.

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Preparation of High Quality Protein Crystals by

Salt Screen. Standard Screen. #. #. Hits. Hits. Precipitant. Precipitant. Salt. Salt. Buffer. Buffer. Like. Like. 73. 75. 11. 8. 30%(w/v) PEG 8000. 30%(w/v) PEG 4000. 0.2 M (NH 4 ) 2 SO 4. 0.2 M Li 2 SO 4. 0.1 M Cacodylate pH 6.5. 0.1 M Tris pH 8.5. H1 #15. H1 #17. 75.

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Target residues - PowerPoint PPT Presentation

Bioinformatics and Molecular Modeling studies of Membrane Proteins

Preparation of High Quality Protein Crystals by

Preparation of High Quality Protein Crystals by