Efficiency of OILIS compared to a chemical coccidiostat and competitor product on zootechnical performances and coccidia

1. Trial report 7_Wisium_SPE-OILIS-TR-RT-YT-RD-2011-EN-V2 Sheep lambs 14 days - slaughter Efficiency of OILIS compared to a chemical coccidiostat and competitor product on zootechnical performances and coccidian oocysts excretion Type of trial: R&D trial Location: Saint Affrique – Agriculture Highschool Date: 2011 Introduction This trial is conducted in order to evaluate the efficiency of OILIS distributed to sheep lambs from 14 days after weaning until their sales. Dosage of OILIS used is 2kg/T of grower feed. Performances obtained with OILIS are compared to a control feed, to a feed containing an anti- coccidian chemical molecule commonly used in prevention (= positive control = decoquinate) and to a competitor alternative (based on vegetal extracts). Materials and methods Housing and animals: 120 Lacaune sheep lambs with a majority of male have been used. They are coming from farrowing beginning the 14 of October and ending on 26 October 2011. Sheep lambs were weaned and allocated in 4 batches of 30 (25 males and 5 females). Sheep lambs are housed in a fattening sheepfold, on straw. Period: From 2011/11/19 to 2012/02/14 (a total of 53 days). Distribution: Sheep lambs are distributed at weaning according to the farming method, their sex, weight, age and ADG during lactation. A definitive distribution occurred after taking into account faecal examination 14 days after weaning (measure of oocysts excretion (coccidiosis)). This in order to have excreting animals (coccidian oocysts) in each group during the transition to tested feed Diets: Starter period Weaning -7 days 7-14 days A-Negative control B - Positive control (Chemical) C - Oilis D - Competitor Measures: Evaluation of zootechnical performances: -Weight at birth, weaning, weaning + 28d, every 3 weeks during the trial and before the departure to slaughterhouse -Feed intake recording Mortality recording Faecal examination for each animal at weaning, weaning + 28d, weaning + 49d and at the departure to the slaughterhouse. Mac Master method was used for counting the oocysts. Transition Growth 14 days - sale Grower feed Grower feed + chemical product = Decoquinate 30mg/kg Grower feed + OILIS SD 2 kg/T Grower feed + competitor 1 kg/T Starter feed + Grower feed Starter feed NEOVIA / WISIUM Talhouët, 56250 Saint-Nolff - France +33 (0)2 97 48 54 54 - contact@wisium.com - www.wisium.com NEOVIA au capital de 30 589 692€ • 636 320 038 RCS Vannes Code APE 1091 Z • Siret : 636 32003800038 Date: 2 Aug. 2018 Page : 1/3

2. Trial report 7_Wisium_SPE-OILIS-TR-RT-YT-RD-2011-EN-V2 Results Mortality A high level of mortality was recorded at the beginning of the distribution of the feed tested. Twelve lambs died and veterinary concluded to a period of infectious myopathy. Weight & ADG WEIGHT (kg) ADG (g/d) At weaning + 15d At the sale (109d) Total finition A - Negative control 15,1 40,3 a 266 ab B - Positive control = Decoquinate 15,1 40,7 a 274 ab C - Oilis 15,6 42,7 b 288 b D - Competitor 15,6 40,3 a 261 a OILIS significantly improves the weight of lambs at the sale compared with the other group (p<0.05). The weight at the sale for OILIS group is improved by 6.0% in comparison with negative control (+2.4 kg). ADG is globally higher for OILIS group in comparison with other products. The difference is significant for group with OILIS in comparison with competitor (+10.1%; p<0.05). Consumption index FCR is not accurate because of high mortality rate. Oocyst excretion On the global period: Oocyst excretion was measured 5 times: 4 times during the trial and one time before at weaning. At weaning, all animals were fed with the same feed and this counting was useful for sheep lambs distribution. The following graph demonstrates that OILIS is able to reduce oocyst excretion 28 days after weaning (in fact, 14 days after the beginning of supplementation of OILIS in feed). According to these results, OILIS allows a good management of coccidian pressure in livestock (-62.7% of oocysts excretion at the peak). - 62,7% NEOVIA / WISIUM Talhouët, 56250 Saint-Nolff - France +33 (0)2 97 48 54 54 - contact@wisium.com - www.wisium.com NEOVIA au capital de 30 589 692€ • 636 320 038 RCS Vannes Code APE 1091 Z • Siret : 636 32003800038 Date: 2 Aug. 2018 Page : 2/3

3. Trial report 7_Wisium_SPE-OILIS-TR-RT-YT-RD-2011-EN-V2 The quantity of oocysts excreted at 28 days after the accounts for the significant presence of coccidia in the sheepfold. Every batch is affected. The faecal examination at weaning allowed guaranteeing the presence of excretory sheep lambs in each box during the inclusion of tested feed. At 28 days post weaning: During the oocysts excretion peak at 28 days post- weaning, sheep lambs having oocysts excretion lower to 10 000 opg/g of feces (considered without any effects) was 13.7% higher for OILIS group in comparison with negative control. Animals with oocysts excretion higher to 100 000 opg/g of feces (level causing generally symptoms), is reduced by 28.9% with OILIS compared to negative control. These data indicate that OILIS allows limiting the excretion of oocysts during the excretion peak and thus, OILIS will be able to prevent and limit diarrhoea period caused by coccidiosis. Conclusion Results of this trial, where OILIS was incorporated at 2kg/T in grower feed from 2 weeks after the weaning until the sale, allow to demonstrate its interest for a better management of coccidian excretion and to optimize grower performances. Zootechnical performances were improved. Sheep lambs weight at the sale were significantly improved by 6% in comparison with negative control (p<0.05). Weight of animal’s in Oilis group is even higher (+5% p<0.05) than the decoquinate group. ADG were numerically higher for OILIS group in comparison with all other solutions tested and control group and significantly higher for OILIS group in comparison with competitor tested (+10.1%; p<0.05). Finally, OILIS obtained the most important reduction of oocysts excretion in comparison with other products tested (-62.7% at the excretion peak at 28 days). Thus, close to 40% of animals in OILIS group have an excretion level considered as low and without any detrimental effect on animals (versus 23% for control group and 24% for competitor group). NEOVIA / WISIUM Talhouët, 56250 Saint-Nolff - France +33 (0)2 97 48 54 54 - contact@wisium.com - www.wisium.com NEOVIA au capital de 30 589 692€ • 636 320 038 RCS Vannes Code APE 1091 Z • Siret : 636 32003800038 Date: 2 Aug. 2018 Page : 3/3