1 / 16

Hands-on Chromatography

Learn about chromatography, a technique used to separate similar compounds based on their adsorption strength. Explore various types of chromatography and hands-on activities to practice the method. Suitable for beginners and students.

sespana
Télécharger la présentation

Hands-on Chromatography

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Hands-on Chromatography Sheryl K. Hoffmann Concordia College CONASTA 60

  2. Definition 1: Chromatography is a technique for separating similar compounds from one another by using differences in the strength of their adsorption on an inert material. Adsorb: hold (molecules of a gas or liquid or solute) to its surface Absorb: swallow up, incorporate, take in Definition 2: Chromatography is a technique which can be used to separate the components of a mixture. The method depends upon differences in the abilities of the components to adhere to a porus solid such as silica gel, called the stationary phase differences in the abilities of the components to be dissolved in a stream of liquid or gas, called the mobile phase, which is passed over the solid. Definitions

  3. Main Types • Paper Chromatography • Thin Layer Chromatography (TLC) • Column Chromatography • High Performance Liquid Chromatography (HPLC) • Gas-Liquid Chromatography

  4. Basic Method

  5. Spotting Technique

  6. Tips • If the spot is large, components start from different origins and will thus blur any separation that would normally occur. • Try a quick dabbing motion, the longer the liquid is in contact with the paper the larger the spot • If the spot is not very concentrated the resulting bands, once spread over a wider area, will be even fainter. • If the solution to be spotted is not very strong, spot several times, being sure to allow the spot to dry in-between, a hair drier will speed up the drying process.

  7. Hands On Activities 1. Practice Spotting 2. Comparison of 1M NaCl & Water in Separating Food Colour 3. Sticks of Chalk 4. Counterfeit Smarties? 5. Comparing Black Inks

  8. Activity 2 – Food Dyes

  9. Activity 3 - Chalk

  10. Activity 4 - Smarties

  11. Activity 5 – Black Pen Ink

  12. Activity 6 – Geranium TLC Paper

  13. Activity 6A – Frozen Spinach

  14. Activity 6A –Spinach

  15. Activity 7 – 2 Columns

More Related