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20 12-iGEM

20 12-iGEM. Southeast university. Break Symmetry. 程 鹏. 周 乐. 周醒驭. 徐小丹. Team members. 凌昕彤. 桂 凯. 陈牧原. 张 俊. 樊雪龙. 高 珅. 贾正阳. Technical Background. Design Scheme. Contents. Experiment. Results && Biobricks &&Potential Work. Biosafety &&Human Practice.

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20 12-iGEM

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  1. 2012-iGEM Southeast university Break Symmetry

  2. 程 鹏 周 乐 周醒驭 徐小丹 Team members 凌昕彤 桂 凯 陈牧原 张 俊 樊雪龙 高 珅 贾正阳

  3. Technical Background • Design Scheme Contents • Experiment • Results && Biobricks &&Potential Work • Biosafety &&Human Practice

  4. Technical Background Main Goal : Engineering bacteria colony to automatically break their own symmetry Create a star!

  5. Whole System Light sensor Antisense FtsZ repress division Toggle switch AHLtransduction

  6. Red light Light receiver express OmpC OmpC express promote Light sensor promoter cIgene cI protein IPTG promote express pcI promoter lacI gene lacIprotein inhibit promote express ultraviolet rays lacIpromoter cIgene cI protein inhibit SAM express promote Lux Igene Lux Iprotein Lac promoter AHL luxR gene luxRprotein express combine promote Luxprpromoter Tby gene transcription FtsZasRNA silencing translate FtsZ mRNA FtsZ protein FtsZ gene transcription

  7. Light Sensor 1 2 Red Light Blue Light

  8. Light Sensor Red Light Cph8

  9. Light Sensor regulated by the ycgF/E system Blue Light Blue Light ycgE Regulatory BLp ycgZ ycgF Binding&&Repress Expression Conformation

  10. Toggle Switch Toggle Switch

  11. AHL-Based Quorum Sensing Activating LuxR lacI+pL LuxI Generating More AHL

  12. Antisense RNA Antisense RNA

  13. Verification for Antisense RNA IPTG=100 IPTG=200 IPTG=0 After 10 hours in 37°C environment

  14. Design Scheme Related Kits: j5526=r11+b34+mRFP+tt i13507=b34+mRFP+tt i13521=tetR+b34+mRFP+tt j04450=lac+b34+mRFP+tt r0011=lacipl r0051=pci r0040=ptetr r0063=luxpl(ahl--) r0062=luxpr(ahl++) r1051=r0051=pcI i732820=b34+LacI+tt j23039=tetR+b34+luxI+tt i13973=pcI+b34+yfp+tt k098991=pcI+b34+gfp+tt p0451=b34+cI+tt k092400=b34+luxi+tt i13202=lacipl+b34+luxI+tt t9002=tetr+b34+luxr+tt+luxpr+b32+gfp+tt f2620=ptetr+b34+luxr+tt+luxpr k145270=pro+b34+luxr+tt j37033=b34+luxr k091204=pro+b34+luxr+tt i0462=b34+luxr+tt k116640=ptetR+b32+luxr+tt j37015=ptetR+b34+luxr+tt+luxpr+b34+luxI+b34+gfp+tt K088006=b33+lacI+lac+b34+cI+b32+gfp+tt+pcI+b34+rfp+tt I763026=ptetR+b34+lacI+tt I763035=ptetr+b34+lacI+b34+gfp+tt p0151=b31+cI+tt p0440=b34+tetr+tt k145205=ptetr+b32+gfp+tt K082034=lac+b34+gfp+tt

  15. Modeling The Simulation Experiment The Macro-Model The Micro-Model -Based on Improved Cellular Automaton -Based on Cellular Automaton

  16. Modeling Approximate Bayesian Computation （ABC）for parameter inference in gene expression model 1. Approximate Bayesian computation (ABC) ABC rejection sampler ABC MCMC 2. Gene expression model

  17. Modeling

  18. Results Results

  19. Verification for Antisense RNA IPTG=100 IPTG=200 IPTG=0 After 10 hours in 37°C environment

  20. others Potential applications Signal Transnduction Biosensor Cell Printing Potential applications

  21. Acknowledgements We would like to thank : Academy of Biomedical Engineering for all these support;

  22. 数学模型 文档 反义 光敏启动 开关 AHL Others

  23. 下一步我们将完成元件的标准化和功能验证，以及各种材料的整理和项目展示工作。 ③ 项目总结 由于缺乏经验，该项目实现效果不如我们预期，但已部分实现。 ② 该项目大部分由我们自主完成（除了非天然序列发夹结构外包合成），技术上不依赖外援。 ①

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