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Immunological Characterization of Nef-Expressing Vaccine Vectors for HIV-1 BF Recombinant Form in Mice

This study focuses on the characterization of vaccine vectors expressing Nef of the BF recombinant HIV-1 circulating form and evaluates the induced immune response in mice. The aim was to analyze subtype-specific immune responses using DNA and MVA vectors expressing NefBF. The vectors were found to be immunogenic in mice with low cross-reactivity against B subtype, showing potential for future HIV vaccine design in regions with a high prevalence of the BF recombinant form.

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Immunological Characterization of Nef-Expressing Vaccine Vectors for HIV-1 BF Recombinant Form in Mice

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  1. Characterization of vaccine-vectors expressing Nef of the BF recombinant HIV-1 circulating form and evaluation of the immune response induced in mice AM.Rodríguez1, G.Schulman1, MF.Pascutti1, F.Ferrer2, G.Turk1, JL. Najera3, D.Mónaco1, G.Calamante2, M.Esteban3, H.Salomón1 and MM.Gherardi1 1Centro Nacional de Referencia para el SIDA, Universidad de Buenos Aires, Buenos Aires, Argentina 2Instituto de Biotecnología,CICVyA-INTA Castelar, Buenos Aires, Argentina 3Departamento de Biologia molecular y Celular, Centro Nacional de Biotecnologia, CSIC, Madrid, España INTRODUCTION In Argentina, epidemiological studies revealed that early predominance of B subtype has diminished due to the emergence of BF recombinants The aim of our work was to analyze subtype specific immune responses For this we developed vectors expressing Nef from CRF12_BF MVA Vector Characterization DNA and MVA VectorsConstruction DNA vector MVA vector DNA Vector Characterization

  2. Cellular immune responses ELISPOT ELISA IFN-g (pg/ml) IFN-g SFC/106 Cross-reactivity against NefB ELISA IFN-g (pg/ml) Conclusion • We developed and characterized DNA and MVA vectors expressing NefBF. • We demonstrated the ability of these vectors to express Nef from murine and human cells. • Both vectors were immunogenic in mice, with low cross-reactivity against B subtype. This work is relevant for the future design of HIV vaccines in our country where there is a high prevalence of BF recombinant form.

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