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Chin Chye Teo , Eng Shi Ong

Evaluation of the extraction efficiency of thermally labile bioactive compounds in Gastrodia elata Blume by pressurized hot water extraction and microwave-assisted extraction. Chin Chye Teo , Eng Shi Ong. Journal of Chromatography A , 1182 (2008) 34–40. Pressurized hot water extraction.

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Chin Chye Teo , Eng Shi Ong

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  1. Evaluation of the extraction efficiency of thermally labile bioactive compounds in Gastrodia elata Blume by pressurized hot water extraction and microwave-assisted extraction Chin Chye Teo , Eng Shi Ong Journal of Chromatography A, 1182 (2008) 34–40

  2. Pressurized hot water extraction A schematic diagram of a self-assembled PHWE system.

  3. 結果 Fig. 1. Effect of different extraction temperatures on the recoveries of (A) GA and (B) VA by PHWE with flow rate: 1.5 ml/min for 50 min. Such a trend was not observed for VA in the same temperature range. The amount of GA extracted was increasing steadily from 60 to 120 °C.

  4. Extraction rate increased with the flow rate of extraction fluid . Fig. 2.Effect of different flow rates on the recoveries of (A) GA and (B) VA by PHWE at extraction temperature: 120 ◦C for 50 min.

  5. Table 1. Comparison of amount of GA and VA extracted from different batches of G. elata Blume using PHWE and heating under reflux with water as extraction fluid The amount of VA extracted was comparable to heating under reflux of different batches of medicinal plants. The higher yield at a higher applied temperature for GA.

  6. 傳統加熱法

  7. 微波加熱法

  8. Microwave-assisted extraction

  9. Closed vessel MAE system

  10. HPLC 幫浦 層析管柱 注射閥 紫外光 偵測器 數據處 理系統 移動相儲瓶 廢液 記錄器 移動相儲瓶、高壓幫浦、注射閥、層析管柱C18 RP(25 cm×I.D 4.6 mm)或同級品。 紫外光偵測器(使用 270 nm)、數據處理系統或記錄器

  11. 參考文獻： (1)尚偉芬等. 天麻藥理作用研究發展. 中草藥 28 (10)：629-632 (1997). (2)吳惠平等. 天麻對實驗性癲癇豚鼠內兒茶酚胺含量的影響. 南京中醫學院學報6 (2)：49 (1990). (3)莫云強. 雲南植物研究 1 (2)：230 (1980). (4)趙國舉等. 人栽與野生天麻中樞藥理作用的比較研究. 武漢醫學院學報 9(1)：91 (1980). (5)石漢基,涂普文,胡家才等：愈風湯天麻湯防治缺血性中風基理的實驗研究. 湖北中醫雜誌 17 (2)：51 (1995). (6)于順龍等. 天麻抗炎效果研究 20 (5)：19-21 (1989). (7)寒冬等. 黃耆、天麻、首烏的延緩衰老作用的實驗研究. 錦州醫學院報 12(4)：171 (1991).

  12. (8)楊菁等. 黃耆、天麻對老年心藏血管疾病患者血中SOD 的影響. 錦州醫學院報 16 (1)：15 (1995). (9)Chu-Rei Wu, Ming-Tsuen Hsieh and Jen Liao. ρ-Hydroxybenzyl Alcohol Attenuates Learning Deficits in the Inhibitory Avoidance Task. Involvement of Serotonergic and Dopaminergic,Chinese Journal of Physiology 39 (4)：265-273(1996).156 (10)Ming-Tsuen Hsieh, Chu-Rei Wu and Chieh-Fu Chen. Gastrodin and ρ-Hydroxybenzyl alcohol facilitate memory consolidation and retrieval, but not acquisition, on the passive avoidance task in rats. Journal of Ethnopharmacology 56 (4) ：45-54 (1997)

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