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This guide provides step-by-step instructions on ensuring sterility in shake flasks by utilizing a PTFE membrane and sealing techniques. Begin by placing the PTFE membrane onto the GL45 connection, followed by positioning a protection ring on top. Next, secure a sealing component on both the membrane and connection points to ensure an airtight seal. Lastly, fix everything securely with a screw cap. Once assembled, squeeze the sensor to initiate the process. Properly following these steps ensures optimal conditions for your experiments.
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Ensure sterility at shake flasks Place PTFE membrane on GL45 connection Place protection ring on membrane Put sealing on both Fix everything with screw cap Squeeze sensor in START!