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A.O.T.F Laser control

A.O.T.F Laser control. Thermally controlled Tellerium Dioxide (TeO 2 ) crystal. Acousto-Optic-Tunable-Filter Principle. Specifications. Polarisation preserving > 70:1 extinction > 85% transmision efficiency blocking > OD 4.0

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A.O.T.F Laser control

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  1. A.O.T.F Laser control • Thermally controlled Tellerium Dioxide (TeO2) crystal Acousto-Optic-Tunable-Filter Principle

  2. Specifications • Polarisation preserving >70:1 extinction • > 85%transmision efficiency • blocking > OD 4.0 • Noise = < 5.0% peak RMS (measured in reflection imaging mode as per std. commissioning spec.) • switching frequency - how quick can you click a mouse button!

  3. AOTF DELIVERS High quality simultaneous multi label imaging • A comparison of classic Neutral Density (ND) attenuation versus AOTF laser control

  4. Spectral separation considerations AOTF allows more control of the laser intensities to allow better assessment of dye co-localisation

  5. FITC channel (522/32) using ND filter CY3 channel (HQ598/40) using ND filter CY5 channel (680/32) using ND filter The FITC channel is bleeding into the CY3 channel in this set-up

  6. 24 Bit merge of FITC/CY3/CY5 using ND filter Cross talk between red and green channels

  7. Fluorogram of FITC & CY3 channels showing FITC bleed through (cross talk)

  8. FITC channel (522/32) using AOTF control CY3 channel (HQ598/40) using AOTF control CY5 channel (680/32) using AOTF control

  9. 24 Bit merge of FITC/CY3/CY5 using AOTF control

  10. Fluorogram of FITC & CY3 channels with AOTF control - displaying little co-localisation

  11. The Comparison Again AOTF imaging ND filter imaging Return to menu

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