1 / 4

ANODE ( + ve)

Plasma Analysis typical class results. ANODE ( + ve). albumin. antitrypsin. fibrinogen. Immunoglobulins (antibodies). Sample loading. CATHODE ( - ve). Initial plasma. Immunoglobulins (pellet 2). Fibrinogen fraction (pellet 1). Albumin fraction (supernatant). Admission.

step
Télécharger la présentation

ANODE ( + ve)

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Plasma Analysistypical class results ANODE (+ve) albumin antitrypsin fibrinogen Immunoglobulins (antibodies) Sample loading CATHODE (-ve) Initial plasma Immunoglobulins (pellet 2) Fibrinogen fraction (pellet 1) Albumin fraction (supernatant)

  2. Admission Lactate Dehydrogenase (LDH) LDH is a tetrameric isozyme containing only two distinct subunits:H(heart, myocardium); M(muscle). The two subunits may be combined in 5 different ways, with particular combinations dominating in some tissues. The different isozymes are readily separated by electrophoresis. Anode (+) LDH1 HHHH H4Heart & RBC LDH2 HHHM H3M Heart & RBC LDH3 HHMM H2M2Brain & Kidney LDH4 HMMM HM3 Cathode (-) LDH5 MMMMM4Liver & Skel. Muscle 12 Hours 24 Hours Example Increase in total (esp. LDH+1 & LDH2) levels in serum following a myocardial infarction. 2 weeks H4 H3M H2M2 HM3 M4

  3. Protein Electrophoresis ANODE (+) CATHODE (+) in situ enzyme stain detects a SINGLE enzyme. Uses substrates and a coupled reaction (to produce a coloured product). General protein stain detects ALL proteins.

  4. Typical LDH Class Results Brain Testes HEART Muscle Lung Kidney LIVER Serum Cathode (-) Anode (+) M4 HM3 H2M2 H3M H4

More Related