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Do white adipose adipocytes express receptors for gut hormones

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Do white adipose adipocytes express receptors for gut hormones

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    2. Do white adipose adipocytes express receptors for gut hormones? Suha S Al-Naimi

    4. Characteristics of GIP and GLP-1

    5. Did you ever wonder why you can tell its meal time without a clock?

    9. Q. Are GLP-1R, GIPR, GHSR and GPR39 present in WAT?

    10. WAT recognised as energy storage organ The discovery of leptin in 1994 revealed an endocrine role in the regulation of energy balance and other physiological processes In addition to leptin WAT secretes a wide range of protein factors - adipokines

    12. Expressions in 2 human WAT depots

    14. GHSR and GIPRs are expressed in both visceral and subcutaneous while GLP-1R was mainly visceral GPR39 mRNA and protein expressed both in omental and subcutaneous fat in obese subjects. Summary of Results (1)

    15. Q. Are GLP-1R, GIPR, GHSR and GPR39 present in the Adipocytes?

    16. Materials and Methods SGBS cells were cultured as in vitro models of human white adipocytes RNA isolation and RT-PCR Western blotting using commercial receptor antibodies

    17. Time course of expression of genes through development of SGBS

    19. In the differentiation process of SGBS adipocyte, GLP-1R and GHSR mRNA expression was late. While GIPR and especially GPR39 mRNA expression was early. Western blot studies confirmed early expression of GPR39 protein in SGBS adipocyte cultures similar to mRNA data. Summary of Results (2)

    20. Q. Gut hormone receptor (s) Expression in Obese human Subjects ?

    21. Gut Hormone Receptor expression in SC WAT depot of obese human subjects

    25. Acknowledgements

    27. Cell culture method

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