280 likes | 403 Vues
This chapter delves into how cells release chemical energy through metabolic reactions, focusing on photosynthesis and cellular respiration. It explains how light energy is converted into glucose (C6H12O6), a process known as photosynthesis, and contrasts it with cellular respiration where stored energy in glucose is converted into usable ATP. Key processes include glycolysis, the Krebs cycle, and the electron transport chain under aerobic conditions, as well as anaerobic respiration techniques like fermentation. This comprehensive overview highlights energy transformation, storage, and usage.
E N D
Chapter 8 How Cells Release Chemical Energy
Metabolic reactions • Photosynthesis • Light energy converted into stored energy (glucose) • CO2 + H2O => C6H12O6 (glucose) + O2 • Endergonic • Cellular Respiration • Stored energy (glucose) converted into useable energy (ATP) • C6H12O6 (glucose) + O2 => CO2 + H2O • Exergonic
Cellular respiration • Aerobic Respiration • Requires oxygen • High energy (ATP) yield • Glycolysis—cytoplasm • Kreb’s Cycle—mitochondrial matrix • Electron Transport System—cristae • Anaerobic Respiration • Doesn’t require oxygen • Organisms without mitochondria • Low energy yield
Aerobic respiration • Step 1—Glycolysis • Glucose (6C) broken down into two PGAL (3C) • PGAL restructured into pyruvate • Produces 2 NADH • Requires 2 ATP to start • Produces 4 ATP • Net gain of 2 ATP • Glucose P-Glucose 2 Pyruvate
Aerobic respiration • Step 2a—Acetyl-CoA • Pyruvate (3C) combines with CoA • Releases CO2 • NAD+ NADH • Forms acetyle-CoA (2C) • 2 Pyruvate => 2 CO2 + 2 NADH
Aerobic respiration • Step 2b—Krebs Cycle • 2 Acetyl-CoA enter • Transfers carbons to oxaloacetate (C4), forming citrate (C6) • Cycles through steps to rearrange citrate • 2 CO2 released • Ends forming oxaloacetate • Cycle starts again • Net gain of 4 CO2, 6 NADH, 2 FADH2, 2 ATP
Aerobic respiration • Step 3—Electron Transfer Phosphorylation • NADH & FADH2 from previous steps start chain • Electrons flow through “chain” of membrane proteins • Each protein then takes H+ from above molecules and pumps them into intermembrane space • This sets up concentration gradient • H+ moves down gradient through ATP synthase • Movement forms ATP from ADP & P (32 net gain) • Ends with electrons passed to O2, combines with H+ to form H2O
Aerobic respiration • If no oxygen, electrons can’t pass on • This backs up to NADPH, so no H+ gradients • No ATP forms, starving cells
Aerobic respiration • Glycolysis • Glucose + 2ATP 4ATP + 2NADH + 2 Pyruvate • Intermediate • 2 Pyruvate 2CO2 + 2NADH + 2 Acetyl-CoA • Krebs Cycle • 2 Acetyl-CoA 6NADH + 2ATP + 2FADH2 • Electron Transfer • 10NADH + 2FADH2 32ATP + 4CO2 + 6H2O • C6H12O6 + 6O2 6H2O + 6CO2 + 36 ATP + heat
Anaerobic respiration • Fermenters • Protists, bacteria • Marshes, bogs, deep sea, animal gut, sewage, canned food • Some die when exposed to O2 • Some indifferent to O2 • Some can use O2, but switch to fermentation when none around
Anaerobic respiration • Glycolysis happens normally • 2 Pyruvate, 2 NADH, 2 Net ATP form • Enough energy for many single-celled species • Not enough energy for large organisms
Alcohol fermentation • Glucose 2 Pyruvate 2 Acetaldehyde + 2 CO2 • NADH + Acetaldehyde Ethanol
Alcohol fermentation • Yeasts • Bread • Beer • Wine
Lactate Fermentation • Glucose Pyruvate Lactate
Lactate fermentation • Can spoil food • Some bacteria create food • Cheese, yogurt, buttermilk • Cure meats • Pickle some fruits & vegetables
Lactate fermentation • Muscle cells • Slow-twitch—light, steady, prolonged activity • Marathons, bird migrations • Many mitochondria • Only aerobic respiration • “dark” meat in birds • Fast-twitch—immediate, intense energy • Weight lifting, sprinting • Few mitochondria • Lactate fermentation • Produce ATP quickly, but not for long • “white” meat in birds
Energy storage • Glucose absorbed through intestines • When glucose level rises, glucose converted to glycogen • Diverts at glucose-6-phosphate in glycolysis
Energy storage • Glycogen is storage polysaccharide • Stores in liver & muscles • With low blood glucose, insulin released • This triggers glycogen to convert back to glucose • If too many carbohydrates/glucose in blood, acetyl-CoA diverted & made into fatty acid
Using fats • Body stores most fats as triglycerides • When glucose levels fall, triglycerides used • Enzymes remove glycerol
Using fats • Glycerol converted to PGAL • PGAL converted to pyruvate as in glycolysis
Using proteins • Happens when eat too many proteins, or when carbohydrates & fats used • Enzymes break down protein molecules • Ammonia (NH3) removed • Leftover carbon backbone split • Forms acetyl-CoA, pyruvate, or intermediate of Krebs cycle • Specific amino acid determines which is formed