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The Role of Genes AT5G48150 and AT2G04890 in Arabidopsis thaliana Seed Development

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The Role of Genes AT5G48150 and AT2G04890 in Arabidopsis thaliana Seed Development

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    1. The Role of Genes AT5G48150 and AT2G04890 in Arabidopsis thaliana Seed Development Jennifer Huynh June 8, 2006 Honors Collegium 70AL Professor Bob Goldberg

    2. Overview

    3. What has been already discovered about the genes?

    4. The GRAS Family Name derives from the three initially identified members GAI, RGA, and SCR Functions range from meristem maintenance to regulation of hormone and light signal transduction PAT1 branch plays role in far red light signaling Homologs found in higher plants Tomato, petunia, lily, rice, barley

    5. The GRAS Family Tree

    6. The GRAS Family Name derives from the three initially identified members GAI, RGA, and SCR Functions range from meristem maintenance to regulation of hormone and light signal transduction PAT1 branch plays role in far red light signaling Homologs found in higher plants Tomato, petunia, lily, rice, barley

    7. Gene One: AT5G48150 Scarecrow-like transcription factor 21 (SCL21) Gene Size: 2355 bp Protein Size: 491 aa

    8. Gene Two: AT2G04890 Phytochrome A signal transduction 1 (PAT1) Gene Size: 2601 bp Protein Size: 414 aa

    9. Where is the gene transcribed into mRNA?

    10. mRNA Accumulation: AT5G48150

    11. mRNA Accumulation: AT2G04890

    12. Is the upstream region of the gene responsible for the transcription of the gene?

    13. Restriction-Digested Plasmid DNA

    14. What happens if the genes are knocked out?

    15. Genotyping Insertion Lines Wildtype: 1508 bp Salk band: 756 bp Found band: 880 bp

    16. Genotyping Insertion Lines

    17. AT5G48150: SALK_064220 Wildtype: 1796 base pairs Salk band: 1740 base pairs Found band: 1739 base pairs

    18. AT2G04890: SALK_146085 Wildtype: 1508 base pairs Salk band: 756 base pairs Found band: 880 base pairs

    19. Nomarski Photographs

    20. Conclusions No mutant phenotype on flowers, leaves, or siliques for either AT5G48150 or AT2G04890 Possibilities Genes not vital for seed development Similar genes exist that compensate Mutant phenotype not visible in experiments More research is necessary

    21. Future Research Perform RT-PCR on homozygous mutants seeds to observe transcription Fuse GFP marker with promoter to observe expression Cross homozygous mutant plants of different knockouts

    22. Acknowledgements Bob Goldberg Anhthu Bui Mike Gavino Jonathan Russell Ria Yagnik Brandon Le Xingjun Wang Tomo Kawashima Jessica Luke HC 70AL Classmates

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