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Fish Cell Biology/Genetics Basics

Fish Cell Biology/Genetics Basics. Dr. Craig Kasper HCC Brandon. Fig. 01-05. Fig. 01-07. Fig. 01-09. Fig. 01-11. Zebra Fish Larval Development. We thank Leon Browder & Laurie Iten (Ed.) Dynamic Development for their gracious use of material seen in slides 11-17.

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Fish Cell Biology/Genetics Basics

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  1. Fish Cell Biology/Genetics Basics Dr. Craig Kasper HCC Brandon

  2. Fig. 01-05

  3. Fig. 01-07

  4. Fig. 01-09

  5. Fig. 01-11

  6. Zebra Fish Larval Development • We thank Leon Browder & Laurie Iten (Ed.) Dynamic Development for their gracious use of material seen in slides 11-17. • We also thank Haffter et al., 1996 and The Company of Biologists for their contributions.

  7. Previous Slide Info… Figure 2. Drawings of zebrafish embryos at 24 hours (A), 48 hours (B,D) and 5 days (C,E) of development. For clarity, the pigmentation pattern is omitted from B and C. It is shown in D and E. Most of the structures that can be seen in a living embryo with a compound microscope are labeled. (From Haffter et al., 1996. Reproduced with permission from The Company of Biologists.)

  8. Gregor Mendel: Quick Review • You might look at possible outcomes for your fish some day. It would be nice to know what to expect, or how you “got what you got…” • Punnett Squares and knowledge of basic genetics will help you determine what traits you select for maybe in your breeding. (At least until you spawn several groups.) More on Punnett Squares shortly…

  9. Introduction • As always we have several new terms. • Genotype: genes of the individual. Your genetic makeup, if you will. • Phenotype: What you look like, or more precisely, what physical manifestations are observed. • Allele: Alternate forms of a gene which are located in the same position on a pair of chromosomes.

  10. Fig. 02-01 • Three paths: • Two results?? • Not really! • Again, new terms… • Dominant allele • (capital letter) • Recessive Allele • (lower case letter)

  11. Alleles on autosomes • Basic Mendelian Genetics • and the Punnett Square • If we “cross multiply,” it • becomes evident that not • all genotypes result in the • same phenotype. • The example at the left is a • single-trait cross. • Notice the expected • phenotypic ratio (3:1). • This will become important • later.

  12. What happens if we • mix it up a bit?? • Same method, different • results. • Now the phenotypic • ratio is 50:50 (Better odds • than you’d get in Vegas!)

  13. Okay, Emeril, let’s… • “Kick it up a notch!!” • Dihybrid crosses • (and beyond) are • handled similarly. • You must be careful • to transfer all your • alleles though!! • Once this is done, • figuring out what • you have can be • challenging.

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