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CLEANSED LIFE + (2014-2015)

CLEANSED LIFE + (2014-2015). Project LIFE12 ENV/IT/000652 Innovative integrated methodology for the use of decontaminated river sediments in plant nursing and road building. Carlos García Izquierdo. CEBAS-CSIC). CLEANSED LIFE PROJECT MEETING PISA (ITALY), FEBRUARY 2016. CEBAS.

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CLEANSED LIFE + (2014-2015)

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  1. CLEANSED LIFE +(2014-2015) Project LIFE12 ENV/IT/000652 Innovative integrated methodology for the use of decontaminated river sediments in plant nursing and road building Carlos García Izquierdo. CEBAS-CSIC) CLEANSED LIFE PROJECT MEETING PISA (ITALY), FEBRUARY 2016

  2. CEBAS Project LIFE12 ENV/IT/000652 CEBAS-CSIC (CLEANSED) Dr. Carlos Garcia Dra. Teresa Hernández Dr. J. Moreno Lcda. Mª Dolores Coll Lcda. Carmen Chocano Lcdo. Manuel Campoy EXPERIMENTAL GREENHOUSES (SANTOMERA-ABARÁN

  3. Project LIFE12 ENV/IT/000652 “ZERO WATES”: a proposalforthefuture New strategiesfornotwastesproduction, or to controlltheproduction with a minorrisk. SUSTAINABILITY (thisisnoteasyforouractivity) Whenwastes are produced: rules forelimination WATES VALORIZATION • RECOVERY (ENERGY) • REUSE (USED FOR OTHER PURPOSES) • RECYCLING: USED FOR ORIGINAL USE • CONVERSION: CONVERT A WASTE TO OTHER PRODUCT

  4. Project LIFE12 ENV/IT/000652 POLLUTED SEDIMENTS : A PROBLEM (CLEANSED SOLUTION) OBJECTIVE: A suatainable management for polluted dredged river sediments. Treatment of polluted sediments to obtaining a valuable material. VALORIZATIONa) Plant nursing: decontaminated sediments (after treatment) could be used as sustrate for plants; b) Sediments could be used for road construction. Sustainable Development (CIRCULAR ECONOMY) We should know:- Quantitative and qualititative sediment characteristics- Decontaminaned technology for polluted sediments- Final use for treated sediments. VALORIZATION OF DREDGED RIVER SEDIMENTS (AFTER TREATMENT)

  5. CEBAS ORGANIC COMPOUNDS (HYDROCARBONS, PESTICIDE, OTHERS..) IN SEDIMENTS: BIOLOGY ELIMINATION • Biostimulation. APPLICATION OF BIOLOGY METHODS (landfarming and phytorremediation) * AERATION * NUTRIENTS Pollutedsediments: LANDFARMING BIOLOGY METHODS (BIOESTIMULATION) FINAL STRUCTURE FOR THE PRODUCT

  6. Project LIFE12 ENV/IT/000652 TREATED SEDIMENT (Landfarming, phytorremediation) • Sustratefornursery • (Agriculturalsoil + sediment) • 2) Materialsfortheroadconstruction.

  7. “TREATED SEDIMENTS” Project LIFE12 ENV/IT/000652 AMENDMENTS FOR SOIL-PLANT SYSTEM Sedimentsforincreasingsoilquality, productivity and fertility EDAPHYC AMENDMENT To increase microbial activity (aerobic and anaerobic): bacterial and fungi. To increase soil structure due to the treated sediment. SEDIMENT FOR SOIL-PLANT SYSTEM

  8. Project LIFE12 ENV/IT/000652 SEDIMENTS ANALYSIS (Contaminated and treated) (landfarming and pytorremediation: T0, T1 and T6) ANALYSIS FROM CEBAS-CSIC ON T6 SAMPLES (samplesaftertreatment)

  9. Project LIFE12 ENV/IT/000652 MIXTURE: SOIL/SEDIMENT/COMPOST/ CEREAL DEBRIS MIXTURE: SOIL/SEDIMENT Ratio: 100 soil -- 0 sediment 100 soil –- 10 sediment 100 soil –- 20 sediment 100 soil –- 40 sediment

  10. Project LIFE12 ENV/IT/000652 CLEANSED PROJECT (B3/C2 Actions) B3. Demonstration of the use of decontaminated sediments as a substrate for plant nursery C.2 Monitoring and validation of the use of decontaminated sediments for plant nursing SOIL/SEDIMENT: Control, T33 and T50 (36 x 2 = 72 samples) Times: T1, T2, T3 and T4 (total samples: 288) ANALYSIS FROM CEBAS-CSIC ON SAMPLES (afterplantation): SOILS AND PLANTS

  11. Project LIFE12 ENV/IT/000652 SOILS ANALYSIS CEBAS GROUP

  12. CLEANSED (CEBAS-CSIC) ANALYSIS ON SOIL SAMPLES OF THE EXPERIMENT: Chemicalanalysis: pH, CE, OM, TOC, water soluble C and N Physicalanalysis: Moisture, water holding capacity, water curves, Toxicologicalanalysis: Cu, Zn, Cd, Cr, Ni, Pb Nutritional and elemental analysis (IONOME): N, P, K, Ca, Mg, Mn, Na, Fe Cationic Exchange Capacity Anions(F-, Cl-, NO2-, NO3-, Br-, SO4-2, PO4-3 ) Plantphytotoxic test (Petri plate). Germinationindex (Lepidiumsativun). Biologicalparameter. Microbialrespiration Project LIFE12 ENV/IT/000652

  13. Project LIFE12 ENV/IT/000652 SAMPLES USED FOR ANALYSIS (CNR—IBIMET+ISE) • FACTORS: • Sediment (0, 33%, 50%) • Soildepth • Sampling (four times) • Plants (threespecies)

  14. Project LIFE12 ENV/IT/000652 pH AND ELECTRICAL CONDUCTIVITY • Soil + TreatedSediment > soilsalinity. • Changeswiththe time of sampling. • No effectbyplantspecies

  15. Project LIFE12 ENV/IT/000652 ORGANIC CARBON • Organiccarbon > Soil + TreatedSediment. • Plantyieldcould be responsible of it (plantdebris). • Differentplants and differentsampling

  16. Project LIFE12 ENV/IT/000652 TOTAL NITROGEN • Total Nitrogen > Soil + Treatedsediment. • 0-15 cm sampling > Total N.

  17. Project LIFE12 ENV/IT/000652 Treatedsediments: > Cation Exchange Capacity.

  18. Project LIFE12 ENV/IT/000652 THE IONOMA: THE KEY OF SOIL FERTILITY ICP-OES equipment Micro and macronutrients were analyzed by ICP-OES (Inductively coupled plasma-optical emision spectrometer) cuantitative determination, is an elemental analysis technic (IONOMIC SERVICE). The “Ionoma” of soils is an important parameters to know the possible importance as biofertilizer, or the pollutant capacity of a treatment. Soil IONOME is indicative of chemical quality of soil

  19. Project LIFE12 ENV/IT/000652 SOLUBLE ELEMENTS -- A Criteria of SOIL FERTILITY: itisimportant to knowthe total quantity of elements, butalsotheavailability of theseelements

  20. Project LIFE12 ENV/IT/000652 Table 18. Soluble Macro and Micronutrients of soil samples at T4. Criteria of SOIL FERTILITY: itisimportant to knowthe total quantity of elements, butalsotheavailability of theseelements

  21. Project LIFE12 ENV/IT/000652 SOLUBLE ELEMENTS • Effecton vegetal specie (Fe and P). • >> Values in lastsampling

  22. Project LIFE12 ENV/IT/000652 Treatedsediments: > Soluble Ca

  23. Project LIFE12 ENV/IT/000652 Control soils: > Soluble Fe

  24. Project LIFE12 ENV/IT/000652 Treatedsediments: > Soluble Mg

  25. Project LIFE12 ENV/IT/000652 Table 22. Macro and Micronutrients of soil samples at T1.

  26. Project LIFE12 ENV/IT/000652 ANIONS • Anions > lastsampling; rootexudatescould be responsibles of it

  27. CLEANSED (LIFE) SOIL BIODIVERSITY: FUNCTIONALITY SOIL RESPIRATION Thedegradation of organicmatteris a property of allheterotrophs, and itsrateiscommonlyused to indicatethelevel of microbialactivity Themetabolicactivity of the sediments, measured as microbial respiration, showdthelowestvalues Project LIFE12 ENV/IT/000652

  28. Project LIFE12 ENV/IT/000652 Table 46. Soil Microbial Respiration of soil samples at different times. • Changesforsoilrespirationwithsampling. • Microbialactivityisnotaffectedbytreatedsediments

  29. Project LIFE12 ENV/IT/000652 • Changesforsoilrespirationwithsampling. • Microbialactivityisnotaffectedbytreatedsediments

  30. Project LIFE12 ENV/IT/000652 PHYTOTOXICITY: PLANT TEST Lolium perenne PHYTOTOXICITY ASSAY SEDIMENT 5 daysincubation 28 ºC and humidity Germinatiónseeds * % germinatedseeds * Roots GerminationIndex

  31. Project LIFE12 ENV/IT/000652 Table 47. Germination Index of soil samples at differents times. • Soil + Sediments: no phytotoxicitywasobserved • Lastsampling >> GerminationIndex

  32. Project LIFE12 ENV/IT/000652 Table 48. Water Retention Capacity of soil samples at differents times (t1 and t2).

  33. Project LIFE12 ENV/IT/000652 PHYSICAL PARAMETER Curves (water holding) on control soil

  34. Project LIFE12 ENV/IT/000652 PLANTS ANALYSIS CEBAS GROUP

  35. CLEANSED (CEBAS-CSIC) ANALYSIS ON PLANT SAMPLES OF THE EXPERIMENT: Chemicalanalysis: TC Toxicologicalanalysis: Cu, Zn, Cd, Cr, Ni, Pb Nutritional and elemental analysis (IONOME): N, P, K, Ca, Mg, Mn, Na, Fe Project LIFE12 ENV/IT/000652

  36. Project LIFE12 ENV/IT/000652 • FACTORS: • Sediment (0, 33%, 50%) • Soildepth • Sampling (four times) • Plants (threespecies) SAMPLES USED FOR ANALYSIS (CNR—IBIMET+ISE)

  37. Project LIFE12 ENV/IT/000652 • Effecton N due to theplantspecie. • * Noteffectdue to thesediment.

  38. Project LIFE12 ENV/IT/000652 • Effecton Al due to theplantspecie. • * Noteffectdue to thesediment.

  39. Project LIFE12 ENV/IT/000652 • Effecton Ca due to theplantspecie. • * Noteffectdue to thesediment.

  40. Project LIFE12 ENV/IT/000652 • Effecton Cu due to theplantspecie. • * Noteffectdue to thesediment.

  41. Project LIFE12 ENV/IT/000652 • Effecton K due to theplantspecie. • * Noteffectdue to thesediment.

  42. Project LIFE12 ENV/IT/000652 • Effecton Mn due to theplantspecie. • * Noteffectdue to thesediment.

  43. Project LIFE12 ENV/IT/000652 • Effecton Pb due to theplantspecie. • * Noteffectdue to thesediment.

  44. Project LIFE12 ENV/IT/000652 • Effecton S due to theplantspecie. • * Noteffectdue to thesediment.

  45. Project LIFE12 ENV/IT/000652 • Effecton Zn due to theplantspecie. • * Noteffectdue to thesediment.

  46. Project LIFE12 ENV/IT/000652 CONCLUSIONS (Ourexperiment) (Soil + TreatedSediment) ON SOILS 1)“Soil +Treated Sediment”:this mixture showed a positive effect on some soil parameters (total organic carbon, soluble organic carbon, cation exchange capacity, soil total nitrogen,…). 2) Electric conductivity, Ca and S total and soluble, increased when treated sediment was used as amendment (maybe due to Ca, Mg,. Sulphates). 3) Fe and P soluble were increased on control soil 4) No change for some soil heavy metals by the use of treated sediments; it is depending of the dose used (particularly for Zn e Cd). 5) No change for microbial activity was noted by the use of treated sediment 6) The use of treated sediment did not affect the phytotoxicity 7) Physical parameters: some positive effect has been observed.

  47. Project LIFE12 ENV/IT/000652 CONCLUSIONS (Ourexperiment) (Soil + TreatedSediment) ON PLANTS 1)“Soil +Treated Sediment”: a positive effect on macro and micronutrients on some plant species was observed. 2) Plant species is the main factor for this study. 3) No change on plant heavy metals by the use of treated sediments was observed 4) pH value (basic) is positive to avoid some risks (e.g., heavy metals accumulation on plants).

  48. PROPOSAL TREATED SEDIMENTS VALORIZATION: itcould be possible WASTE TO RESOURCE TREATED SEDIMENTS AS SUSTRATE: -- Exhaustiveanalysisfortreatedsediments ( (MONITORING) -- Doses and Management (MAIN POINT) -- To knowthe vegetal specie and thesoil -- Annualmonitoring of soil-plantsystem TO AVOID RISK FOR ENVIRONMENT AND PEOPLE INFORMATION----RESEARCH----RULES

  49. Project LIFE12 ENV/IT/000652

  50. Project LIFE12 ENV/IT/000652

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