Masaryk U niver s it y Enantis, s.r.o. VOP-026 Šternberk, s.p., division VTÚO Brno C PN , s. r.o.

1. ENZYMATIC DE C ONTAMINA T I O NO F S U L F U R M U S T A R D Masaryk University Enantis, s.r.o. VOP-026 Šternberk, s.p., division VTÚO Brno CPN, s.r.o.

2. W a r f a r e a g e n t s u l f u r m u s t a r d • used for the first time in 1917 near Belgium city Ypres • easily penetrates to variousmaterials • makes burn marks and blisters due to skin contacts • military use banned by international convention • easy to prepare from common chemicals

3. T e c h n o l o g y d e v e l o p m e n t • Czech Republic – development of enzymatic decontamination mixturefor sulfur mustard prioritized by NATO • research projects funded by Ministry of Defence 2004 – 2006 and Ministry of Education 2005 – 2011 • degradation of sulfur mustard by enzymes dehalogenases demonstrated • protection of intellectual properties – Czech patent CZ 298287 • technology transfer to practice – biotechnology spin-off Enantis

4. H a l o a l k a n e d e h a l o g e n a s e s

5. H a l o a l k a n e d e h a l o g e n a s e s

6. T o x i c s u l f u r m u s t a r d Cl Cl S

7. N o n t o x i c a n a l o g u e Cll Cl O

8. C o m p u t e r m o d e l l i n g

9. C o m p u t e r m o d e l l i n g

10. C o m p u t e r m o d e l l i n g

11. C o m p u t e r m o d e l l i n g sulfur mustard bis(2-chloroethyl) ether

12. E x p e r i m e n t a l t e s t s

13. E x p e r i m e n t a l t e s t s

14. P a t e n t - p r o t e c t e d c o n c e p t

15. R e p o r t s i n s c i e n t i f i c j o u r n a l s

16. T e c h n o l o g y t r a n s f e r t o p r a c t i s e development of decontamination mixture optimisation of protein production large-scale industrial production product commercialization development of new and improved products

17. P r o d u c t d e v e l o p m e n t a n d t e s t i n g • testing of dehalogenases in decontamination aerosols(Pacific Northwest National Laboratory – USA) • testing of dehalogenases in polymer particles(Foster & Miller, US Army – USA) • testing of dehalogenases inmicroemulsions(Defence Science and Technology Laboratory – Great Britain) • testing of dehalogenases in skin decontamination cream(Bundeswehr Institute of toxicology and pharmacy – Germany) • NATO testing of enzyme decontamination on aircraftinterriers

18. C o n s o r t i u m o f p a r t n e r s Masaryk Universityand Enantis, s.r.o. • 14 years of research on dehalogenases • > 40 genes, >100 protein variants • know-how from 29 research stages • modern laboratories and facilities for protein engineering E N Z Y M E D I S C O V E R Y AND O P T I M I Z A T I O N • isolation and construction of new enzymes • genetic breeding of enzymes with improved catalytic properties

19. C o n s o r t i u m o f p a r t n e r s Masaryk University and Enantis, s.r.o. VOP-026 Šternberk, s.p., division VTÚO Brno • applied research and development of decontaminations and defence against warfare of mass destruction • personal and infrastruture based on 50 years of experience T E S T I N G O F E N Z Y M E S W I T H W A R F A R E A G E N T S • study of warfare chemicals with extreme toxic properties • compatibility testing of individual components for decontamination mixtures

20. C o n s o r t i u m o f p a r t n e r s Masaryk University and Enantis, s.r.o. VOP-026 Šternberk, s.p., division VTÚO Brno CPN, s.r.o. • Production of pharmaceuticals and cosmetics • large-scale industrial fermentations and down-stream processing P R O C E S S S C A L E – U P A N D P R O D U C T I O N • optimization of enzyme production, pilot-scale and full production • development of protective cream for skin decontamination

21. E x p e r i e n c e s w i t h t e c h no l o g y t r a n s f e r • high-quality basic research: • systematic and selective financial support • investment into human resources • competitive infrastructure • financial support and consulting for protection of intellectual properties • business consulting and administrative support • collaboration with local industrial partners

22. E x p e r i e n c e s w i t h t e c h no l o g y t r a n s f e r • high-quality basic research: • systematic and selective financial support • investment into human resources • competitive infrastructure • financial support and consulting for protection of intellectual properties • business consulting and administrative support • collaboration with local industrial partners

23. E x p e r i e n c e s w i t h t e c h no l o g y t r a n s f e r • high-quality basic research: • systematic and selectivefinancial support • investment into human resources • competitive infrastructure • financial support and consulting for protection of intellectual properties • business consulting and administrative support • collaboration with local industrial partners

24. CTT A c k n o w l e d g e m e n t s

25. E x c u r s i o n t o l a b o r a t o r i e s • Jan Josef Loschmidt • (1821 – 1895)

26. E x c u r s i o n t o l a b o r a t o r i e s • established 1994,funded by industry, 1 laboratory • until today 18 grants, 6 international • laboratories 5,instrumentation 1 mil EUR • in parallel 12 research projects • know-how from 29 research stages, 11 visitors • courses Bioinformatics and Biocatalysis

27. E x c u r s i o n t o l a b o r a t o r i e s 3 doctors, 10 doctoral students, 5 diploma students, 1 technician