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Aspartate aminotransferase (EC. 2.6.1.1)

Aspartate aminotransferase (EC. 2.6.1.1). Oleh : Dewi Kurnia (20511029) Dina Soes Putri (20511037). Isi Presentasi. Pendahuluan Fungsi Struktur Mekanisme katalisis Isolasi dan pemurnian Aplikasi Modifikasi Genetik. Pendahuluan. Struktur 3D. Struktur tia p s ubunit . Fungsi.

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Aspartate aminotransferase (EC. 2.6.1.1)

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  1. Aspartateaminotransferase(EC. 2.6.1.1) Oleh: DewiKurnia (20511029) Dina SoesPutri (20511037)

  2. IsiPresentasi • Pendahuluan • Fungsi • Struktur • Mekanismekatalisis • Isolasidanpemurnian • Aplikasi • ModifikasiGenetik

  3. Pendahuluan

  4. Struktur 3D

  5. Strukturtiap subunit

  6. Fungsi • AST berperan dalam proses gliseroneogenesis hati • Secara umum, AST mengkatalisis interkonversi dari aspartat dan α-ketoglutarat menjadi oksaloasetat dan glutamat. Reaksi sederhananya sbb: • Asp +α-ketoglutarat ↔oksaloasetat +Glu

  7. Mekanismekatalisis

  8. Isolasidanpemurnian

  9. Aplikasiindustri • Digunakansebagaipenandaadanyakerusakanselhatiakibatpaparanpelarutorganikkarsinogen (CCl4, CHCl3, bahankimipadadry clean, dll) • Sebagaialatinvestigasipada pre-klinisobatbarudenganpengukurankadar AST dalam serum

  10. ModifikasiGenetik • In-frame deletion 3 asam amino gen GOT1 padaurutannukleotida 1165-1167 (AAC) mengakibatkanpenghapusanAsn 389. • Urutannukleotidatersebutdiketahuisebagaiconserved region cASTpadamamalia • Delesiinimengakibatkanpenurunanaktifitasenzim AST secarasignifikan

  11. DaftarPustaka • http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.1 • http://www.chem.qmul.ac.uk/iubmb/enzyme/EC2/6/1/1.html • http://www.wikipedia/aspartate_transaminase.htm • http://omim.org/entry/138180 • http://www.hepatitis-central.com/hcv/labs/liverenzymes.htm • http://www.medfriendly.com/aspartateaminotransferase.html • www.rscbi.org/pdb/explore/pudmed.do?structureId=3II0

  12. TerimaKasih

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