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Recap of iGEM 2012 Weekly Meeting: Challenges and Future Directions in Synthetic Biology

This summary covers the key points discussed during the Purdue iGEM meeting on June 15, 2012. The team reviewed previous week's challenges, including troubleshooting transformation failures and bad sequencing results for AIDA-1. Research initiatives focused on primer construction, organization of committees, and planning for upcoming experiments on Curli and FimA-H constructs. The meeting emphasized the need for collaboration, with various committees outlining their to-do lists, and members encouraged to read relevant literature to deepen their understanding of synthetic biology principles.

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Recap of iGEM 2012 Weekly Meeting: Challenges and Future Directions in Synthetic Biology

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Presentation Transcript

  1. Purdue iGEM 2012 Meeting Friday 6/15/12

  2. Recap of the Past Week’s Work • Trouble shooting with transformations • Discovery of bad sequencing in AIDA-1 • Primer construction • Order Gibson Plasmids • Meeting with Janie and Dr. Litster • Research on experiments to characterize constructs

  3. Things to Discuss this Week • Curli, Ag43, AIDA-1, FimA-H • Transformation failures • Primer designs for Gibson Assembly • Organization of growing group • Committees? • Use of Wiki

  4. Plan for the Next Week • Trouble Shoot with transformations • Continue transformations • Set up controls and experimental plans to get data to optimize setup using JMP • Read another paper?

  5. Journal Review! • Everyone read a paper on either syn bio or an area related to the project • Wrote short summary to be uploaded to drop box • Present that info now 

  6. Committees • Experimental Design – Statistics/make product work • Mrudula* • Chris • Arthi • Modeling - JMP • Max* • Haefa • Wiki (Notebook/Admin) • Rachel* - collaborating • Background – Sean • Committees update as well • Characterizing – parts specific • Amanda* • Chris • Rachel • Wetlab Work • OmpA-Sil • August* • Haefa • AIDA-1 • Rubeena* • Purchasing • Peter* – cc him on all e-mails so he can keep a list • Human Practices • Peter* • Max* • Project Planning • Namita

  7. To Do Next Week • Wetlab to do (Rubeena/August): • Transformation trouble shooting - if still not working call iGEM to get new cells • Check the concentration of the DNA in the wells with nanodrop (should get 20) • Contamination with cells we have? • Re-competent the cells from Janie • Check the concentration of Amp • Get one transformation to work before continuing – check with Soo’s protocol from workshop • Call iGEM TODAY!!!!!!!!! • Grow biofilms! • Ask Eric McLamore about how best to make biofilm/bioreactor • Detatchment assay • Make Primers for Curli for the biobrick plasmid • Rachel – give to Wetlab committee (Rubeena) • Purchase the primers • Read a paper - everyone • Start rotating describing the project to the group – set up rotation – Amanda for Friday August next Friday • Order OmpA-Silicatein construction to be synthesized! • Bring forms to Tuesday meeting for purchasing • Committees meet and set up To-Do lists and meetings within committee

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