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160 anti-CD antibodies are robotically arrayed in a nitrocelllulose-coated glass slide, then the

STEPS in Preparing a PBMC Array. 160 anti-CD antibodies are robotically arrayed in a nitrocelllulose-coated glass slide, then the surface is “blocked” with BSA. 1. 10 6 PBMCs are added to the slide surface and allowed to settle. 2.

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160 anti-CD antibodies are robotically arrayed in a nitrocelllulose-coated glass slide, then the

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  1. STEPS in Preparing a PBMC Array 160 anti-CD antibodies are robotically arrayed in a nitrocelllulose-coated glass slide, then the surface is “blocked” with BSA 1 106 PBMCs are added to the slide surface and allowed to settle 2 Antibodies ‘capture’ only PBMCs that express corresponding CD antigens. Washing removes the rest 3 PBMCs bind at different CD antibody spots in proportional to the number of cells bearing that epitope, and the number of surface CD epitopes per cell 4

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