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Proteomics: 2D Gel

Proteomics: 2D Gel

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Proteomics: 2D Gel

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  1. Proteomics: 2D Gel

  2. Proteome analysis 장치 (2D)

  3. Root Proteome Nodule Proteome

  4. Table I. Proteins identified in soybean root and nodule through MALDI-TOF/MS & LC-MS/MS

  5. 단백질의 질량 스펙트럼 효소 질량분석기 MS spectrum 단백질 Protein database Peptide sequences Protein id A B A B

  6. Proteomics: MALDI-Mass

  7. Mass spectrum vs. database Mass spectrum database Protein A Protein B Protein C

  8. 탄뎀 질량 스펙트럼(MS/MS spectrum) energy 효소 1st MS 2nd MS 단백질 MS spectrum Peptide sequence Protein id Protein database Tandem MS spectrum

  9. R1 O R2 O R3 O R4 COOH H2N C C N C C N C C N C H H H H H H H 탄뎀 질량 스펙트럼Peptide fragmentation x3 y3 z3 x2 y2 z2 x1 y1 z1 a1 b1 c1 a2 b2 c2 a3 b3 c3

  10. 2D data 분석