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Analysis of lytic proteins pinholin and Rz/Rz1. Sue Parks and Lawangin Khan. Lytic Cassette. S. R. Rz. Rz1. Comparative analysis of Trans-Membrane domain Motifs in pinholin proteins. Two Domains in the Pinholin Protein. Figure 1D: Phage S21 reading frame.
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Analysis of lytic proteins pinholin and Rz/Rz1 Sue Parks and Lawangin Khan
Lytic Cassette S R Rz Rz1
Comparative analysis of Trans-Membrane domain Motifs in pinholin proteins
Two Domains in the Pinholin Protein Figure 1D: Phage S21 reading frame Pang et al.(2010) Mutational Analysis of S21 pinholin. Molecular Biology, 76(1): 66-77.
Traditional Holin Model TM2 TM1 TM3
Text TMD1 TMD2
Motifs MXXM TMD1 TMD2
Comparative analysis of lysis protein, Rz1 cleavage sites • Lawangin Khan
Localization? Why Cleave? Cleave site
Cleavage site conserved? Determining cleavage site • Determined by LipoP, (www.cbs.dtu.dk/services/LipoP/)
Relative Consensus and Position • Krupovic’s lipobox consensus: L(A/S)(G/A)C