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Nematode Extraction Methods: Foliar Extraction Techniques

Nematode Extraction Methods: Foliar Extraction Techniques. Kavitha Govindasamy Jimmy R.Rich Maria L .Mendes. A Plant-Parasitic Nematode. Sampling for Nematodes. Sampling and extracting for nematodes serves two purposes Diagnosis a current problem Predict a future problem.

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Nematode Extraction Methods: Foliar Extraction Techniques

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  1. Nematode Extraction Methods:Foliar Extraction Techniques Kavitha Govindasamy Jimmy R.Rich Maria L .Mendes

  2. A Plant-Parasitic Nematode

  3. Sampling for Nematodes Sampling and extracting for nematodes serves two purposes • Diagnosis a current problem • Predict a future problem

  4. Objectives of sampling and extraction Identify the nematode Population estimation Diagnose a nematode disease Make management decisions Regulatory purposes Research and surveys

  5. Some Common Nematode Extraction Methods • Foliar samples Punching and incubating Scissors technique • Soil samples Baermann funnel Modified Baermann funnel • Root Samples Jar incubation and sieving Blending and sieving

  6. Foliar Extraction Techniques:Using a punch or scissors • Principle Nematodes migrate out of the leaves when the lesions are soaked in water

  7. Materials Required Hole punch or scissors Petri dish or other container

  8. Cut leaf tissue and place in water

  9. Punch the lesions and place tissue disks in water

  10. Take samples near healthy tissue

  11. Incubate 24 hours and observe under microscope

  12. Acknowledgments • Janete Brito, Department of Plant Industry, Gainesville, Florida • Frank Woods, Nematode Assay Lab, University of Florida, Gainesville, Florida • Joey Orajay, Entomology and Nematology Department, University of Florida, Gainesville, FL

  13. For additional information or comments, please contact: • Kavitha_nem@yahoo.com • jimmyr@ufl.edu • mlmendes@ufl.edu

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