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Attack of the Superbugs Lab Introduction

Attack of the Superbugs Lab Introduction. Mrs. Stewart Medical Interventions. Superbugs. What is a “superbug”? Bacteria that have become stronger and less responsive to antibiotic treatment. How can bacteria share resistance?. This lab uses 2 types of Bacteria:. E. coli I

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Attack of the Superbugs Lab Introduction

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  1. Attack of the Superbugs Lab Introduction Mrs. Stewart Medical Interventions

  2. Superbugs • What is a “superbug”? • Bacteria that have become stronger and less responsive to antibiotic treatment

  3. How can bacteria share resistance?

  4. This lab uses 2 types of Bacteria: • E. coli I • contains a gene found on the chromosomal DNA coding for streptomycin resistance • E. coli II • contains a gene found on the plasmid DNA coding for ampicillin resistance.

  5. Predict • What will happen when the E. coli I strain is mixed with the E. coli II strain?

  6. Lab Materials: 4 growth plates LB Agar LB + Str LB + Amp LB /Str /Amp *LB Agar = growth medium for bacterial cells

  7. Lab Day 1: • Obtain 2 of each type of growth plate • Streak E. coli I on 4 plates • Streak E. coli II on 4 plates • Incubate overnight *These will be the confirmation plates

  8. Evaluate • What are we confirming?

  9. Lab Day 2: • Observe confirmation plate results • Prepare “mix” plate • You will literally be mixing the two types of bacteria together onto one plate • Incubate overnight

  10. Evaluate • Why are we mixing them together?

  11. Lab Day 3: • Use new “mixed” bacterial cultures and spread onto the 3 types of antibiotic plates

  12. Predict • What would you expect to see on these growth plates? • Why?

  13. FRIDAY – Lab Day 4 • You must come in at some point during the day Friday to observe and record your results • Remember: Pictures of lab results will be required in your lab reports *Growth plates will be discarded at 3:10 pm on Friday. Any results not recorded will be lost.

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