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Cellular Respiration Investigation

This investigation explores how temperature influences the rate of cellular respiration in germinating and non-germinating peas. By measuring oxygen consumption through a respirometer, we can understand the relationship between temperature and respiration rates. We will utilize the general gas law and account for variables such as pressure and volume during the experiment. The collected data will be analyzed for trends, allowing discussion of potential experimental errors, their impacts, and corrective measures. The study aims to explain results while relating them to ATP production in plants.

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Cellular Respiration Investigation

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  1. Cellular Respiration Investigation

  2. Question How does temperature affect the rate of respiration in germinating and non-germinating peas?

  3. C6H12O6 + 6O6 6CO2 + 6H2O + 686 kcal of energy/mole of glucose How could the rate of cellular respiration be measured?

  4. Measuring Oxygen Consumption General Gas Law PV = nRT P = Pressure of the gas V = Volume of the gas n = number of molecules of gas R = gas constant T = temperature of the gas (in ⁰K) If P and T are constant? If V and T are constant? If n and T are constant? If T changes and n is constant?

  5. Measuring Oxygen Consumption Removing Carbon Dioxide CO2 + 2KOH K2CO3 + H2O *Since any carbon dioxide produced will be removed, any change in the volume of gas will be directly related to changes in oxygen.

  6. Apparatus Used

  7. Respirometer Trials

  8. Prediction and Hypothesis Record in your notebook…

  9. Procedure Record Procedure and Data Collection Table in your notebook as a pre-lab entry.

  10. Analysis • Make a graph of your data. Show each variable as one line. • Describe the data. • Discuss errors. - What were they? - How did they affect the data? - How could they be corrected?

  11. Conclusion • Compare actual to expected results. • EXPLAIN the results for each variable. Use your knowledge of the equation for respiration and the Gas Law. -Explain why water moves in a respirometer’s pipette. -Compare the amounts of oxygen consumed by germinating corn seeds at room temperature and in the ice bath. -Compare the results for dry and germinating seeds. -What are some processes in plants that require ATP? • Further Questions • Real-life application

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