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Investigating Gene Expression in SH-SY5Y and SK-N-BE(2)c Cells Using Antibody-Free Probes

This study explores the expression of various markers, such as β-Actin and BSAP, in SH-SY5Y and SK-N-BE(2)c cell lines using time-course analysis at different hours (0, 8, 24, 48, 72, 94) without the use of antibodies. We employ Octamer transcription factors and Pax-5-free probes to assess gene regulation and cellular responses. Results indicate the stability of β-Actin and differential expression of BSAP, providing insights into neuronal differentiation and potential therapeutic targets in neuroblastoma.

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Investigating Gene Expression in SH-SY5Y and SK-N-BE(2)c Cells Using Antibody-Free Probes

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  1. B IMR-32 KCN-69n LA-N-1 SH-SY5Y No antibody No antibody No antibody No antibody Oct  Actin  Actin  Actin  Actin  BSAP  BSAP  BSAP  BSAP Oct Oct Oct Oct Pax-5 Free probe C SK-N-BE(2)c Oct BSAP  BSAP  Actin Probe 8 24 48 72 94 0 8 24 48 72 94 (hr RA) 0 Pax-5 Free probe

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