Mohiuddin _Figur e S1

1. Mohiuddin_FigureS1 A 2 h 10 min B 5 h 2 h 10 min

2. AvrII AvrII BamHI P3 AID P4 P1 Mohiuddin_FigureS2 MARCO TR C Human cells (293T cells) P5 P2 A B MfeI AvrII BamHI MfeI Genetically modified Retroviral expression vector Helper plasmid (pCLampho) TOPO Cloning TOPO Cloning TR MARCO WT cells with chimera, △FEN1 cells with chimera & △REV3 cells with chimera Viral supernatant TR MARCO pTOPO BamHI AvrII AvrII MfeI WT, △FEN1 & △ REV3 DT40 cells Digest with BglII & EcoRI Digest with BamHI & MfeI BamHI BamHI Digest with AvrII pTOPO pTOPO C C T A G G G G A T C C MARCO TR IRES IRES IRES GFP GFP GFP Digest with BamHI & AvrII Digest with BamHI & AvrII BamHI MfeI SI Nuclease BamHI BglII MARCO TR BglII EcoRI EcoRI BglII EcoRI MARCO MfeI AvrII C G G C TR MARCO TR AvrII BamHI Ligate BamHI MfeI pMSCV pMSCV pMSCV pTOPO TR MARCO MARCO TR Ligate pTOPO AvrII BamHI MfeI MARCO TR pTOPO

3. Mohiuddin_FigureS3

4. 24 h after exposure 24 h after exposure Mohiuddin_FigureS4 WT A WT + Beads Side Scatter Side Scatter WT with Long Chimera Count Count WT with Long Chimera + Beads WT with Short Chimera WT with Short Chimera + Beads Forward Scatter Green Fluorescence Red Fluorescence Forward Scatter Green Fluorescence Red Fluorescence

5. 3 h after exposure 3 h after exposure Mohiuddin_FigureS4 (continued) WT B WT + Beads Side Scatter Side Scatter WT with Long Chimera Count Count WT with Long Chimera + Beads WT with Short Chimera WT with Short Chimera + Beads Forward Scatter Green Fluorescence Red Fluorescence Forward Scatter Green Fluorescence Red Fluorescence C WT with Short Chimera WT with Long Chimera WT Without Beads With Beads

6. Table S1: List of primers used to make chimeric receptor. Name Sequence P1 (+) ATTAGGATCCATGGATCATGCCAGAGCAGCATTGTCTAAC P2 ( -) ATTACCTAGGAAGATGACAGGCGGTCCTTGAATCTC P3 (+) ATTACCTAGGGACACAAAGGAGAACGCGGTCC P4 (+) ATTACCTAGGTGGAAATCTTTCACCAAGGGTCCTGG P5 (- ) ATTACAATTGTCAGACACACTCCACGCCGGCGTC