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In vitro pH Optimization of AOMT Activity with Cyanidin 3-O-Glucoside and Delphinidin 3-O-Glucoside

Evaluate pH optimum of AOMT using cyanidin 3-O-glucoside and delphinidin 3-O-glucoside, ranging pH 4.5-9.4 with specific buffers.

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In vitro pH Optimization of AOMT Activity with Cyanidin 3-O-Glucoside and Delphinidin 3-O-Glucoside

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  1. Figure S3. A nanoKat / mg protein pH values B nanoKat / mg protein pH values Figure S3.In vitro pH optimum of AOMT using (A) cyanidin 3-O-glucoside and (B) delphinidin 3-O-glucoside. The pH dependence of AOMT activity was assessed in the pH range 4.5 – 9.4 using MES (4.5 – 6.5), PIPES (6.6), HEPES (7.0 – 7.5) and Tris-HCl (7.5 – 9.4).

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