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Optical Measurement Techniques in Tissue Analysis

This study explores the interactions of light with materials, focusing on absorption, transmission, and scattering methods in tissues. It delves into the applications of light interactions in nature, medical, and biological fields and discusses the challenges in studying tissue scattering and absorption. The Kubelka-Munk equations are examined alongside conditions for their application, with an emphasis on developing a better understanding of light propagation in multiple scattering media. Experimental procedures involving tissue preparation and optical measurements are detailed, highlighting the calculation of optical constants and factors for tissue analysis.

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Optical Measurement Techniques in Tissue Analysis

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  1. Optical Measurment In Tissue Kamal Abu Shindi 20063092006 Advisor: Mohammad AL-Akhras Department of Physics in Jordan University Science & Technology “JUST” Jordan – Irbid December- 2007

  2. Introduction:- • 1- The Interact of light with material take multiple method Absorption Transmission Scattering • 2-show the Applications to the Interact of light in nature and medical and biological 3-show The difficulty to study a scattering and absorption to a tissue • 4- develop study the propogation of light in amultiple scattering medium • 5- schuster (1905)→ Dreosti(1930) → Kubelka- Munk (1931) →Kubelka(1948)

  3. The Kubelka – Munk {K – M} equations . 1-The condition to applay {K – M} equations : • a- the elementary volume elements is chosen such that only single scat- • tering take place • b- the scattering non-coherent c- Isotropic 2-If three condition applied then the absorbed and scattring light is linearly dependent on thickness 3- we let the sample is persumed to have one dimensional geometry ,Area much larger than thickness .

  4. J I0 0 • dI= - K.I.dx – S.I.dx + S.J.dx • - dJ = - K.J.dx – S.J.dx + K.I.dx • Boundary condition : at X=0 →I=I0 and X=d →J=0 • and Td = I(d)/I0 Rd = J(o)/ Io • by solve equation we obtain dx +x I

  5. S= (1/Cd) coth-1 (1+T^2-R^2)/2R C • K/S = [ (1 + Rd^2 – Td^2)/ (2Rd) ] – 1 • WhereC = √k/S ((k/S) +2) • From these equation we can calculate (K,S) from Measurment (R,T,X)

  6. Transmission and optical density • OD = - Log T (d) • Beer’s Low I(x)=I0 exp(-µx) • At x=1HVL then I(x) =0.5* I0 = I0 *exp(-µx) • 1HVL= 0.693/µ • Penetration Depth π= π0 exp( -x/δ)

  7. Experimental procedure • 1-we make a tissue from a powder potato mix with pure water at concentration 16.3gm powder / 1 liter water • 2-revolve the mix with heat for 20 minutes • 3-input the slap among empty 1mm • 4-keep the cup in refrigerator. • 5-after freezing the mix we cut the tissue for circular slide • 6-keep the side in dark and cold place • 7-we go to take date from UV spectra-meter about [ wave length (λ) , absorption (od)]. • 8-and we go to X-ray spectrometer to take data.

  8. UVGlass

  9. UVAgar

  10. UVAgar+Glass

  11. X Agar

  12. xAgar+Glass

  13. Conclusion and summary • 1- we can calculate attenuation coefficient and half value layer from transmission and thickness at constant wave length . • 2-we can Calculate the scattering and absorbing factors from transmission and reflection diffusion • 3-The one-dimensional diffusion approximation implement for fast calculation of microscopic optical constants from diffuse transmission and reflection measurement.

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