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Microbial Diversity in Drain Hole Waters of the Henderson Mine

Microbial Diversity in Drain Hole Waters of the Henderson Mine. John R. Spear Jason Sahl Division of Environmental Science and Engineering Colorado School of Mines, Golden, CO jspear@mines.edu. $125 x 10 6. $125 x 10 6. Amount spent in the U.S. on marshmallows in 2005. What Is Life?.

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Microbial Diversity in Drain Hole Waters of the Henderson Mine

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  1. Microbial Diversity in Drain Hole Waters of the Henderson Mine John R. Spear Jason Sahl Division of Environmental Science and Engineering Colorado School of Mines, Golden, CO jspear@mines.edu

  2. $125 x 106

  3. $125 x 106 Amount spent in the U.S. on marshmallows in 2005

  4. What Is Life?

  5. A “Map” of Life Euryarchaeota Euryarchaeota Crenarchaeota Crenarchaeota You are here N.R. Pace, Science, 1997

  6. Molecular Method Env. Sample Extraction Bulk DNA/RNA PCR Community rRNA/rDNA Cloning rRNA/rDNA Clones Sequencing rRNA/rDNA Sequences Databases Phylogenetic Trees

  7. 7150’ DH-3 Data Fungus Sulfate Reducing Bacteria

  8. 7025’ DH-1 Data Fungus Sulfate Reducing Bacteria

  9. 7025’ DH-1-Post Packer Data Fungus Sulfate Reducing Bacteria

  10. 7025’ DH-4-Post Packer Data Fungus Sulfate Reducing Bacteria

  11. DH Chemistry

  12. HM

  13. Conclusions • Potential for 3 new groups (phyla) of Bacteria!! • Something is happening with N!! • Community shift pre- verses packed- drill holes. • Surprising diversity for a subsurface. • Low abundance of both Archaea and Eucarya! • Publish?

  14. Thanks To: • My Start-up money…. • Team Bio--Tom K., Jeff M., Alexis T., Betsy S., Jeff W., Kevin M. • HUSEP Folks • Henderson Mine and Chip deWolfe

  15. “If it wasn’t for the optimist, the pessimist would never know…. how happy he wasn’t.”

  16. Henderson, New Drill Core • Plan, to drill a core into the HUSEP facility area • For some portion of that: • Take a segment for biological consideration • Sterile? BEADS + Tracer • Core collection--on site • Core sample sections to Socorro, CSM, CU • Continue drilling to depth

  17. Henderson Mine, 2 Nov 05

  18. Molecular Method Env. Sample Contact Slide Extraction Whole-Cell Hybridization (FISH) Bulk DNA/RNA PCR Nucleic Acid Community rRNA/rDNA Nucleic Acid Probes Hybridization Cloning Probe Design rRNA/rDNA Clones Sequencing rRNA/rDNA Sequences / Databases Phylogenetic Trees = Method= Result

  19. DH-2, DH-3 + ???? To Do…. Water Water Anoxic H2O out = 1.5’ PVC Pipe Inserted with Glass and/or Fe Growth Slides

  20. DH-1 Water Chemistry + Cultures Species Concentration Si4+ 0.05 mM Ca2+ 8.7 mM Mg2+ 1.3 mM Al3+ 4.8 mM Na+ 7.9 mM Sr2+ 0.04 mM K+ 1.0 mM FeT 1.5 mM Mn2+ 21 mM F- 12 mM Cl- 0.15 mM NO2- Not detected NO3- 1.0 mM Br- Not detected PO43- Not detected SO42- 48 mM Culture efforts: Swanner & Templeton, CU: DH-1 3 (Fe + Mn) DH-3 3 (Mn) DH-4 2 (Fe + Mn) DH-1 at 7150’, pH 5.8

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