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This study explores ZFN-mediated gene disruption in rat embryos and its application in creating Rainbow transgenic mice. By expressing combinations of three distinct fluorescent proteins (XFPs), up to 160 colours can be generated, enhancing multicolour neuronal labelling. The independent recombination of transgenes leads to a diverse spectrum of fluorescence, allowing detailed visualization of neural structures such as motor nerves and the hippocampal dentate gyrus. This innovative approach is a significant advancement in understanding neuronal connectivity and function.
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Fig. 1 ZFN-mediated gene disruption in rat embryos. A M Geurts et al. Science 2009;325:433-433 Published by AAAS
Combinatorial expression of three distinct fluorescent proteins can generate a large spectrum of colours Ten distinct colours can be generated by expressing a trimeric combination of three different XFPs. In Brainbow mice, this outcome would result if three copies of a trichromatic transgene, each recombined independently Nat Rev Neurosci. 2008 June; 9(6): 417–422
Multicolour neuronal labelling in Brainbow transgenic mice • Nat Rev Neurosci. 2008 June; 9(6): 417–422. A A motor nerve innervating ear muscle. b | An axon tract in the brainstem. c | The hippocampal dentate gyrus. In the Brainbow mice from which these images were taken, up to ~160 colours were observed as a result of the co-integration of several tandem copies of the transgene into the mouse genome and the independent recombination of each by Cre recombinase (see FIG. 2c). The images were obtained by the superposition of separate red, green and blue channels.
