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Recap of Purdue iGEM Weekly Meeting: Transformations, Experimental Design, and Project Updates

This recap summarizes the Purdue iGEM meeting held on June 19, 2012. Key discussions included updates on transformations and minipreps, project control details, the design of experiments based on recent methods, and planning for the upcoming week's activities. Team members coordinated tasks for the WIKI meeting and discussed the synthesis of OmpA using Gibson assembly and 3A assembly methods. Collaboration was emphasized, as members planned meetings and experimentation to advance their research objectives.

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Recap of Purdue iGEM Weekly Meeting: Transformations, Experimental Design, and Project Updates

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  1. Purdue iGEM 2012 Meeting Tuesday 6/19/12

  2. Recap of the Past Week’s Work • Transformations • Minipreps • Not ordering all of OmpA – Silicatein • Experimental Design Methods • Eric McLamore • Reading Papers

  3. Mini Prep

  4. Plan for the Next Week • 3A Assembly • Start designing experiments based on methods from today • Split up work for WIKI

  5. Meeting Notes: • Eric McLamore – what exactly is your project? More details so we could use it as a control • Quorum system • Does he have a control system • What is his area of expertese? • Steve is back in his office – wants to talk to him • Human Practices are talking to him Thurs • Call and find out if we can present our project thus far • E-mail stats professors • How do we want to synthesize OmpA? • Just making OmpA with Gibson the rest is using 3A • Sean made the primers in the dropbox

  6. Plan for the Next Week • Tuning the system with different strains of promoter and RBS • In biobrick workshop – ask Soo • Just use the ones we have • Mini prep Tuesday • Transforming Wed and another Mini prep • Transformation #2 Thurs • Mini prep #2 Fri • Weekend Transformation #3 • Mon or Tues done with Transformation • Max and Mrudula meet with Janie about varialbes for model in JMP • Send to Amanda, Rubeena, Rachel to start designing experiments to run

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