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Fluorescence resonance energy transfer

Fluorescence resonance energy transfer<br>

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Fluorescence resonance energy transfer

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  2. 2020/10/8 Protein Interaction Service - Creative BioMart Yeast two-hybrid: Genome-wide screening of protein interactions by co-expression of bait with protein libraries. Membrane-based yeast two-hybrid: Based on the split-ubiquitin protein complementation assay and detects protein interactions directly at the membrane. Mammalian two-hybrid: Allows rapid and convenient analysis of protein-protein interactions in transfected mammalian cells.  Phage display technology: Obtain optimal protein binding by immobilization of an antigen on magnetic beads, and screening against a phage display library. Surface plasmon resonance (SPR): The versatile label-free BIACORE technique allows detecting and monitoring of interactions in real time. Protein array: This technique is a high-throughput method used to track the interactions and activities of proteins, and determining function on a large scale. https://www.creativebiomart.net/Protein-Interaction-Service.htm 2/8

  3. 2020/10/8 Protein Interaction Service - Creative BioMart Co-Immunoprecipitation (CO-IP): In vivo technique that detects naturally formed protein complex. Pull-downs: Confirm the existence of a protein-protein interaction predicted by other research techniques and identify previously unknown protein-protein interactions as an initial screening assay. Bio-layer interferometry (BLI): Quantitative and qualitative characterization of biomolecule interactions. CLIP-Seq technology: RNA-binding landscape mapping of interacting proteins or RNA modification sites on a genome-wide scale. Fluorescence resonance energy transfer (FRET): Test the direct interaction in vitro of two candidate proteins, suitable for both wild type and mutant proteins. Biomolecular fluorescence complementation (BiFC): This technique is based on the principle that two nonfluorescent fragments of a fluorescent protein dissected at appropriate site are brought together and reconstructed to fluorescence, depending on the association or interaction between the protein fused to each fragment. Far-western blotting: This method employs non-antibody proteins to probe the protein(s) of interest on the blot. Protein binding site mapping: Locate the binding sites with a high degree of confidence and predict the druggability of those sites. https://www.creativebiomart.net/Protein-Interaction-Service.htm 3/8

  4. 2020/10/8 Protein Interaction Service - Creative BioMart  Benefits working with us: Thorough expertise in protein-protein interactions Flexible offer adapted to your requirement High-value scientific assistance Highly sensitive homogeneous assay We will be glad to discuss details of intended interaction studies with you and develop experimental strategies/methods tailored to your requirement. Please get in contact with Creative BioMart for more information or a detailed discussion. Welcome to contact us or send an email to info@creative-biomart.com for project quotations and more detailed information. Online Inquiry Name: Your Name * Phone: Phone * E-mail Address: Email Address Organization: Organization https://www.creativebiomart.net/Protein-Interaction-Service.htm 4/8

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