Analysis of Mutated Recombinant Proteins via SDS-PAGE: Purification Procedure

Jan 12, 2025

10 likes | 77 Vues

This figure presents SDS-PAGE results of mutated recombinant proteins in the purification process. Fractions eluted from a heparin column were loaded and revealed by Coomassie staining using molecular weight markers.

adele-harding

Télécharger la présentation

Analysis of Mutated Recombinant Proteins via SDS-PAGE: Purification Procedure

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

Fig S1 IN 143R/97A IN 143C/97A IN 97A IN 143C IN 143R IN WT (kDa) MW 100 70 55 35 IN 25 15 10 Figure S1.SDS-PAGE of mutated recombinant proteins used in this study. Proteins obtained in the purification procedure were analyzed on SDS-PAGE and revealed by coomassie staining. 5 µL fractions eluted from heparin column were loaded on the gel. MW, molecular weight markers (PageRuler Prestained protein ladder plus, Fermentas).