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Microscope

Microscope. 02. Carrying a Microscope. A. B. Storing The Microscope. Return the lowest power objective in place Wrap the cord around the base Return dustcover. Cleaning the Microscope. Use lens paper on all glass parts of the microscope.

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Microscope

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  1. Microscope 02

  2. Carrying a Microscope A B

  3. Storing The Microscope • Return the lowest power objective in place • Wrap the cord around the base • Return dustcover

  4. Cleaning the Microscope • Use lens paper on all glass parts of the microscope. • Clean oil immersion lens with chemicals provided by your instructor

  5. Parts of the Microscope • Ocular • Body tube • Nosepiece • Objective lenses (10, 40, 100x) • Arm • Course adjustment knob • Fine adjustment knob • Stage • Stage clips • Aperture • Diaphragm • Light • Base

  6. Microscope Parts • Ocular • Body tube • Head • Revolving nose piece • Objective • Arm • Stage • Diaphragm • Lever to move stage clip • Course adjustment • Fine adjustment • Light source • Base

  7. Using the microscope • Always observe using the LOWEST POWER objective first. • Focus using the COARSE ADJUSTMENT KNOB to bring the object into focus. Bring the object into sharp focus by using the fine adjustment knob. • Focus, and then move to a higher power objective, if needed. • Use only the FINE ADJUSTMENT KNOB when using the HIGHEST (longest) POWER OBJECTIVE. • Keep both eyes open to reduce eyestrain. • Determine total magnification of the object by multiplying the power of the ocular (10x) the power by the power of the objective.

  8. Preparing a slide • Using a pipet or dropper, add a drop of water or another solvent to a clean microscope slide. Then, place the specimen in the water. • Place the edge of a coverslip on the slide so that it touches the edge of the water. • Slowly lower the coverslip to prevent the formation of air bubbles.

  9. Oil Immersion Lens 100x 40x

  10. Animal Cell

  11. Plant Cell

  12. Cheek Cell

  13. Elodea

  14. Epidermal Onion Cell

  15. Mitosis

  16. Mitosis Prophase Late Prophase Interphase

  17. Mitosis Telophase Metaphase Anaphase

  18. Mitosis

  19. Dispose Biological material

  20. Dispose Sharps

  21. Clean Up

  22. Microscope Parts Quiz

  23. Microscope Quiz • Which objective uses oil? • If your ocular is 10x and your objective is 40x what is the total magnification of your image? • Why do you need a cover slip and how do you avoid an air bubble? • Where do you dispose slides?

  24. P. 45 lab manual sperm smooth muscle blood Simple squamous epithelial

  25. Cheek Cell 0.9% saline

  26. Cheek Cell • Scrape inside cheek • Swirl toothpick with cells in saline • Place a small drop methylene blue on saline saline

  27. Cheek Cell • Place coverslip • Observe under microscope • Draw what you see • Clean up

  28. Cheek Cell

  29. Pond water Yeast

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