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This study presents supplementary data pertaining to PCR primer sequences utilized in the analysis of human embryonic stem cells (ES cells). The sequences are derived from various references, highlighting key research that elucidates the mechanisms of stem cell proliferation and differentiation. Notable studies include the effects of Basic FGF and BMP signaling on ES cell maintenance, as well as the generation of insulin-secreting cells from pancreatic endoderm derived from human ES cells. This resource serves as an essential tool for researchers in the field of regenerative medicine.
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Supplementary Table 1. PCR primer sequences used in this study
Supplementary Table References 1. Xu RH, Peck RM, Li DS, et al. Basic FGF and suppression of BMP signaling sustain undifferentiated proliferation of human ES cells. Nat Methods 2005;2: 185-190. 2. Kroon E, Martinson LA, Kadoya K, et al. Pancreatic endoderm derived from human embryonic stem cells generates glucose-responsive insulin-secreting cells in vivo. Nat Biotechnol 2008;26: 443-452. 3. Xiao L, Yuan X, Sharkis SJ. Activin A maintains self-renewal and regulates fibroblast growth factor, Wnt, and bone morphogenic protein pathways in human embryonic stem cells. Stem Cells 2006;24: 1476–1486.