PCR Primers Utilized in the Study

Jan 12, 2026

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This document provides detailed information on the PCR primers employed in our research. It includes the sequences, concentrations, and specific conditions under which these primers were optimized for the study. The use of these primers is crucial for the amplification of targeted DNA regions, allowing for accurate analysis and validation of results. The methodologies adopted ensure reproducibility and reliability of the PCR results, contributing to the overall robustness of the study.

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dna amplification
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PCR Primers Utilized in the Study

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Supplementary Material 2. PCR primers used in this study.

Supplementary material

analytical method transfer using equivalence tests with reasonable acceptance criteria and appropriate effort: extension of the ISPE concept. L. Kaminski § , U. Schepers § , H. Wätzig* §both authors equally contributed to this article. Supplementary material. Introduction .

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Supplementary data Table 1. PCR primers used to amplify TP53 Exons from genomic DNA

Supplementary data Table 1. PCR primers used to amplify TP53 Exons from genomic DNA. Arya et al. Supplementary data Table 1. MTT/ OD 570nM. Time/Days. Arya et al., Supplementary data Figure 1. 5 μ M Nutlin-3. 0.5 μ M Nutlin-3. DMSO. Untreated. DNA content. UM-SCC-5 (het).

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Supplementary Material

Supplementary Material . Lecture 2. Inheritance . If a gene has a two or more variants then these are called alleles; alleles are the result of mutations in gene. The presence of such alleles is the basis of differences between members of a species; Tall/dwarf [in certain plants] .

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Supplementary Material

Supplementary Material . C. E. A. B. D. F. Supplementary Figure 1. C. E. A. B. D. F. Supplementary Figure 2. C. E. A. B. D. F. Supplementary Figure 3.

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Supplementary Material 2

Supplementary Material 2.

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Supplementary Table 1. PCR primer sequences used in this study

Supplementary Table 1. PCR primer sequences used in this study. Supplementary Table References 1. Xu RH, Peck RM, Li DS, et al. Basic FGF and suppression of BMP signaling sustain undifferentiated proliferation of human ES cells. Nat Methods 2005;2: 185-190.

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Supplementary material

Supplementary material An transcriptional repressor ERF transcription factor confers improved drought tolerance in rice and regulates genes that are preferentially located on chromosome 11 Joungsu Joo 1 , Hae Jong Choi 1 , Youn Hab Lee 1 , Yeon-Ki Kim 2 , and Sang Ik Song 1

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Material Library Used in this FEA

Young’s modulus E, MPa. Poison’s ratio n. Thermal k, w/m-K. CTE a, /K. HDI. 30e3. 0.30. 0.26. 17e-6. Silicone tape 3M 3719. 2. 0.36. 0.24. 100e-6. Silicon sensor. 110e3. 0.30. 141. 2.6e-6. Silicon ROC. 110e3. 0.30. 141. 2.6e-6. Silicone glue NEE001. 2. 0.36. 0.24.

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Supplementary Table I . Gene-specific primers used for quantitatative real-time PCR

Supplementary Table I . Gene-specific primers used for quantitatative real-time PCR.

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Supplementary Table 2: Nucleotide sequence of primers used for PCR amplification

Supplementary Table 2: Nucleotide sequence of primers used for PCR amplification. Gene Accession no. Sequence cDNA Annealing Product Source

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Table S1. Primers used for semiquantitative RT-PCR

Table S1. Primers used for semiquantitative RT-PCR. 4. 4. 10. 10. 3. 3. 10. 10. 93.4. 96.6. 2. 2. 10. 10. 1. 1. 10. 10. 0. 0. 10. 10. 0. 1. 2. 3. 4. 0. 1. 2. 3. 4. 10. 10. 10. 10. 10. 10. 10. 10. 10. 10.

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Additional file 4 – Primers used for PCR analysis of transformants

Additional file 4 – Primers used for PCR analysis of transformants.

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Supplementary Material 2

Supplementary Material 2 Linear regression between RIN and relative non-normalized quantities of candidate reference genes.

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Supplementary table 1: Primers used for PCR amplification

Primer name. Sequence 5`-3`. Accession Number. ChiF. ACCAAGCTACTCGCAAGAGG. CD485880. ChiR. CGGAAGCGCAGTAAGATGA. GluF. CATTGATATGACCTTGATCG. CD486342. GluR. GTGAGATATCCCTTGGATTG. PR1F. TGCCCAAGACTCACAACAAG. CK988133.1 (Patil et al. 2005). PR1R. GGCCTTCTCATTAACCCACA. Hist3F.

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Table S1. Primers and Probes Used in RT-PCR and qMSP

Supplementary Information. Table S1. Primers and Probes Used in RT-PCR and qMSP. Probe. Gene. Forward Primer. Reverse Primer. RT-PCR. GAPDH. GATATCTGGGTCATCGCTTC. TGAGTTTCCGTGAGGCTGAG. EMX2. GATATCTGGGTCATCGCTTC. TGAGTTTCCGTGAGGCTGAG. GAPDH EMX2. qPCR. Hs99999905_m1 Hs00244574_m1.

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Supplementary Table A1. List of siRNAs used in this study

Supplementary Table A1. List of siRNAs used in this study. Supplementary Table A2. List of primers used for real time PCR. Supplementary Table A3. Lysis buffers used for Western blot. Supplementary Table A4. List of antibodies used for Western blot analysis. *. GAPDH. b -actin. srp-14.

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Supplementary Table 1 . List of primers used for real-time PCR analysis

Supplementary Table 1 . List of primers used for real-time PCR analysis. --------------------------------------------------------------------------------------------------------------------------------------- Gene Forward primer Reverse primer

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Supplementary Table 1 Sequences of quantative RT-PCR primers

Supplementary Table 1 Sequences of quantative RT-PCR primers. Supplementary Table 2 Body weight, plasma parameters and blood pressure of E-DNI  B mice. Supplementary Table 3 Body weight and plasma parameters of E-DNI  B mice. Supplementary Figure 1. Lung. Aorta. Macrophages.

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Supplementary Material.

Similar Folds with Different Stabilization Mechanisms: The Cases of Prion and Doppel Proteins S. Colacino, G. Tiana, G. Colombo. Supplementary Material. Pages 2-7 Graphs showing the components of eigenvectors 2 and 3 from each simulation.

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Supplementary Table. Antibodies used in this study

Supplementary Table. Antibodies used in this study.

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Supplementary Material

Supplementary Material Effect of the transposable element environment of human genes on gene length and expression Daudi Jjingo, Ahsan Huda, Madhumati Gundapuneni , Leonardo Mariño -Ramírez and I. King Jordan. A. B. TE fractions.

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Table S2. Sequences of primers used for RT-PCR

Supplementary Materials. Table S1. Features of patients with acute myocardial infarction or unstable angina and control subjects. AMI: acute myocardial infarction. * ANOVA test after the values were logarithm-transformed, # Chi-square test. ACEI: angiotensin convert enzyme inhibitors.

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